Ordering Information
- : AB5062P
- : 50 μg
- : 0.5 mg/mL
Anti-Vesicular GABA Transporter (VGAT)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, M, H, Mk | ELISA, IF, IH, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Vesicular GABA Transporter (VGAT)
Trade Name:
Chemicon (Millipore)
Specificity:
Specific for Vesicular GABA Transporter (VGAT). Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter. The immunogen peptide shows no significant homology with other known proteins.
Molecular Weight:
57 kDa
Epitope:
C-terminus
Immunogen:
A 17 amino acid peptide sequence near the carboxy terminal region of rat VGAT (McIntire, S., 1997).
Background Information:
Vesicular GABA Transporter (unc-47) homologue is a 525 amino acid long protein belonging to the family of neurotransmitter transporter (1). VGAT functions in the uptake of GABA and glycine into synaptic vesicles. Since the protein is bound to synaptic vesicles, VGAT antibodies are excellent markers of presynaptic GABAergic and glycinergic neurons.
Species Reactivity:
- Rat
- Mouse
- Human
- Monkey
Species Reactivity Note:
Rat. Antibody cross-reactivity with various species is not known. The immunogen sequence is 100% conserved in mouse, human and monkey.
Application Notes:
Immunohistochemistry/Immunofluorescence:
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.
ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.
Optimal working dilutions must be determined by the end user.
2-20 µg/mL of a previous lot was used on paraformaldehyde fixed sections.
ELISA:
1:10,000 – 1:100,000 dilution of a previous lot was used on ELISA, using 50-100 ng of control peptide (Cat. No. AG379) per well.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The expected molecular weight is approximately 55-60 kDa.
Optimal working dilutions must be determined by the end user.
Control:
Brain tissue, rat retina lysate
Quality Assurance:
Evaluated by Western Blot on Rat retina lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected Vesicular GABA Transporter on 10μg of rat retina lysates.
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS with 0.1% BSA and 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- SLC32A1
- VIAAT
- VGAT
- bA122O1.1
- hVIAAT
Alternate Names:
Vesicular Inhibitory Amino Acid Transporter
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunofluorescence
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.
UniProt Summary:
FUNCTION: SwissProt: Q9H598 # Involved in the uptake of GABA and glycine into the synaptic vesicles.
SIZE: 525 amino acids; 57415 Da
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY : Retina. Expressed throughout the horizontal cells or more specifically at the terminals.
SIMILARITY: SwissProt: Q9H598 ## Belongs to the amino acid/polyamine transporter 2 family.
SIZE: 525 amino acids; 57415 Da
SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY : Retina. Expressed throughout the horizontal cells or more specifically at the terminals.
SIMILARITY: SwissProt: Q9H598 ## Belongs to the amino acid/polyamine transporter 2 family.
Brand Family:
Chemicon
Product Name:
Anti-Vesicular GABA Transporter (VGAT)
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 μg
Format:
Affinity Purified
Host:
Rabbit