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Ordering Information

  • : AB5204-50UL
  • : 50 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I

Species Reactivity Key Applications Host Format Antibody Type
M, R IHC, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes type I alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

SPECIES REACTIVITIES: It is expected that the antibody may also react with human due to sequence homology. Other species have not been tested.
Immunogen:
Purified peptide SP11 (Noda et al. 1986) from 465-481 of alpha subunit of rat type I voltage-gated sodium channel (VGSC), conjugated to KLH with gluteraldehyde (Harlow & Lane 1988).
Species Reactivity:
  • Mouse
  • Rat
Application Notes:
All procedures that are going to receive a full-length protein should be performed at 4C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

Western blot: 0.5 to 1.5 μg/mL (1:200-1:600) using ECL

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Control:
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Storage Conditions:
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • SCN1A
  • SCN1
  • HBSCI
  • Nav1.1
  • FEB3
  • NAC1
  • SMEI
  • GEFSP2
Alternate Names:
Nav1.1; SCN1A
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunohistochemistry
  • Western Blotting
Entrez Gene Summary:
The vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials, chiefly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and 2 smaller auxiliary beta subunits. Functional studies have indicated that the transmembrane alpha subunit of the brain sodium channels is sufficient for expression of functional sodium channels (Goldin et al., 1986 [PubMed 2429308]; Isom, 2002 [PubMed 11779698]).[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P35498 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
SIZE: 2009 amino acids; 228972 Da
SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: P35498 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
DISEASE: SwissProt: P35498 # Defects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a severe form of generalized epilepsy with febrile seizures. It is a rare disorder characterized by normal development before onset, seizures beginning in the first year of life in the form of generalized or unilateral febrile clonic seizures, secondary appearance of myoclonic seizures, and occasionally partial seizures. It is associated with ataxia, slowed psychomotor development, and mental decline. & Defects in SCN1A are the cause of familial hemiplegic migraine 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia and phonophobia. Age at onset ranges from 6 to 15 years. Some patients may manifest seizures during infancy. & Defects in SCN1A are the cause of familial febrile convulsions 3 (FEB3) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients. Inheritance pattern is autosomal dominant.
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
Brand Family:
Chemicon
Product Name:
Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Affinity Purified
Host:
Rabbit
Applications:
Detect Sodium Channel using this Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I validated for use in IH & WB.

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