Ordering Information
- : AB5340P
- : 50 µg
- : 1 mg/mL
Product Images
Chicken anti-CART (Catalog Number AB5340P) photo shows neurons (cell bodies) in the rat hypothalamic arcuate nucleus having strong CART immunoreactivi...
Anti-Cocaine and Amphetamine Related Transcript
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ELISA, WB, IH | Chicken | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Cocaine and Amphetamine Related Transcript
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Cocaine and Amphetamine Related Transcript (CART). CART has been found to control satiety modulating the actions of two key regulators of food intake, leptin and NPY. Starvation decreases CART levels in the arcuate nucleus (Kristensen et al. 1998). Obese animals have virtually no CART. CART inhibits both normal and starvation-induced feeding, and completely inhibits NPY-induced feeding. Immunoneutralization of CART by administration of anti-CART resulted into higher food intake suggesting that CART is an endogenous regulator of food intake.
Immunogen:
Mouse/rat CART peptide (55-102).
Isotype:
IgY
Species Reactivity:
- Mouse
- Rat
Application Notes:
Western blotting: 1-10 μg/mL using ECL.
Immunohistochemistry: It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.
ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide per well.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: It has been reported that the antibody will recognize CART in neurons (cell bodies) from the rat hypothalamic arcuate nucleus.
ELISA: 1:10,000-1:50,000 using 50-100 ng control peptide per well.
Optimal working dilutions must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CARTPT
- CART
Alternate Names:
CART/CARP
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
CART appears to have roles in reward, feeding, and stress, and it has the functional properties of an endogenous psychostimulant (Kuhar et al., 2002 [PubMed 12147208]).[supplied by OMIM]
Key Applications:
- ELISA
- Western Blotting
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: Q16568 # Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus. It promotes neuronal development and survival in vitro.
SIZE: 116 amino acids; 12829 Da
SUBCELLULAR LOCATION: Secreted (Potential).
TISSUE SPECIFICITY: Hypothalamus. Found in neurons of the ventrolateral part of the arcuate nucleus, in the external zone of the median eminence, and also found in terminals in the periventricular part of the paraventricular nucleus.
DISEASE:SwissProt: Q16568 # A defect in CART is associated with reduced resting energy expenditure and cosegregates with obesity phenotype.
SIMILARITY: SwissProt: Q16568 ## Belongs to the CART family.
SIZE: 116 amino acids; 12829 Da
SUBCELLULAR LOCATION: Secreted (Potential).
TISSUE SPECIFICITY: Hypothalamus. Found in neurons of the ventrolateral part of the arcuate nucleus, in the external zone of the median eminence, and also found in terminals in the periventricular part of the paraventricular nucleus.
DISEASE:SwissProt: Q16568 # A defect in CART is associated with reduced resting energy expenditure and cosegregates with obesity phenotype.
SIMILARITY: SwissProt: Q16568 ## Belongs to the CART family.
Qty/Pk:
50 µg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Chicken
Product Name:
Anti-Cocaine and Amphetamine Related Transcript
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody