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Ordering Information

  • : AB5382
  • : 50 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

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Western Blot Analysis:
Representative lot data.
Mouse liver lysate was resolved by electrophoresis, transferred to PVDF membrane and probed ...

Anti-Nitric Oxide Synthase II Antibody

Species Reactivity Key Applications Host Format Antibody Type
M, R ICC, IHC, WB Rabbit Serum Polyclonal Antibody
Description:
Anti-Nitric Oxide Synthase II Antibody
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Inducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with rat nNOS and eNOS by Western blot and immunohistochemistry.
Molecular Weight:
131 kDa
Epitope:
C-terminus
Immunogen:
C-terminal 14 amino acid peptide from mouse macrophage nitric oxide synthase.
Background Information:
Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: N­terminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and C­terminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaM­binding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation.
Species Reactivity:
  • Mouse
  • Rat
Species Reactivity Note:
Mouse and rat. Reactivity to human iNOS is approximately 1%.
Application Notes:
Immunohistochemistry:
1:5000 (ABC) dilution of a previous lot was used on tissue sections (rat liver).

Immunocytochemistry:
1:50,000 dilution of a previous lot was used on cultured cells.

Western blot:
1:5,000 (ECL). The antibody has been used successfully on rat liver. It is not recommended for use on rat brain extracts since unidentified bands may be present.

Optimal working dilutions must be determined by end user.
Control:
Macrophages, liver lysates
Quality Assurance:
Evaluated by Western Blot on Mouse liver lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected INOS on 10 µg of Mouse liver lysates.
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal serum in buffer containing liquid 0.05% sodium azide.
Storage Conditions:
Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • NOS2A
  • iNOS
  • HEP-NOS
  • INOS
  • NOS
  • NOS2
  • EC 1.14.13.39
Alternate Names:
  • NOS II
  • iNOS
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Entrez Gene Summary:
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
UniProt Summary:
FUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
SIZE: 1153 amino acids; 131117 Da
SUBUNIT: Homodimer. Binds SLC9A3R1.
TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
Brand Family:
Chemicon
Product Name:
Anti-Nitric Oxide Synthase II Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Serum
Host:
Rabbit
Applications:
Anti-Nitric Oxide Synthase II Antibody is an antibody against Nitric Oxide Synthase II for use in IC, IH & WB.

Product Resources

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