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Ordering Information

  • : AB5535
  • : 100 µg
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.
  • Product Family Information

Product Images

Western Blot Analysis:
HepG2 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-SOX-9 (1:500 dilution of t...

Western Blot Analysis:
Representative image from a previous lot.
HepG2 lysate (Lane 1) and PC3 lysate (Lane 2) were resolved by electrophor...

Anti-Sox9 Antibody

Recommended Replacement for: AB5809
Species Reactivity Key Applications Host Format Antibody Type
H, M, R IHC, WB Rabbit Affinity Purified Polyclonal Antibody
Description:
Anti-Sox9 Antibody
Replaces:
AB5809
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Sox9.
Molecular Weight:
56-65 kDa
Immunogen:
Synthetic peptide from human Sox9.
Background Information:
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
Species Reactivity:
  • Human
  • Mouse
  • Rat
Species Reactivity Note:
Human, rat and fetal mouse. Other species have not yet been tested.
Application Notes:
Western Blot:
1:500 dilution of a previous lot ~65kDa band

EMSA supershift

Optimal working dilutions must be determined by the end user.
Control:
Embryonic tissue, Adult chondrocytes.
Quality Assurance:
Routinely evaluated by Western Blot on L6 cell lysates.

Western Blot Analysis: 1:2000 dilution of this lot detected SOX-9 on 10 μg of L6 cell lysate
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • SOX9
  • CMPD1
  • CMD1
  • SRA1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunohistochemistry
  • Western Blotting
Entrez Gene Summary:
The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
UniProt Summary:
FUNCTION: SwissProt: P48436 # Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
SIZE: 509 amino acids; 56137 Da
SUBCELLULAR LOCATION: Nucleus (Potential).
DISEASE: SwissProt: P48436 # Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male- to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
SIMILARITY: SwissProt: P48436 ## Contains 1 HMG box DNA-binding domain
Brand Family:
Chemicon
Product Name:
Anti-Sox9 Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Target / Localization:
Early marker of cranial neural crest derived chondrocytes
Format:
Affinity Purified
Host:
Rabbit
Applications:
Anti-Sox9 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Sox9 & has been validated in IHC & WB.

Product Resources

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