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Product Images
Chicken anti-GFAP (Catalog Number AB5541). Localization of GFAP in cultured rat neonatal forebrain cells grown in tissue culture for 15 days. The blue...
Hippocamppal derived rat glial cells stained with Chicken Anti-GFAP and Mouse Anti-Vimentin.
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Anti-Glial Fibrillary Acidic Protein
| Reatividade à Espécie | Aplicações Essenciais | Hospedeiro | Formato | Tipo de Anticorpo |
|---|---|---|---|---|
| H, M, R, B, Po | IC, IH, IH(P), WB | Chicken | Affinity Purified | Polyclonal Antibody |
Número Uniprot:
UniProt Summary
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease i ... see more »
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease i ... see more »
Número Genbank:
Entrez Gene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Description:
Anti-Glial Fibrillary Acidic Protein
Background Information:
Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Alternate Names:
GFAP
Specificity:
Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Immunohistochemistry(paraffin):
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.
Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.
Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.
Optimal working dilutions must be determined by the end user.
STAINING PATTERN:
The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
Representative images from a previous lot. Pictures of Optimal Staining With Citrate Buffer Epitope Retrieval: Human Brain.
Immunocytochemistry:
1:200-1:1,000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Western blot:
1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.
Immunohistochemistry:
1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.
Optimal working dilutions must be determined by the end user.
STAINING PATTERN:
The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
Species Reactivity:
- Human
- Mouse
- Rat
- Bovine
- Pig
Species Reactivity Note:
Human, rat, and mouse. Expected to cross-react with bovine and porcine. Other species have not yet been tested.
Immunogen:
Purified bovine GFAP.
Molecular Weight:
55 kDa
Control:
Astrocytes, astrocytomas, neural stem cells.
Presentation:
Purified chicken polyclonal in buffer containing PBS containing 5 mM sodium azide.
Storage Conditions:
Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- GFAP
- FLJ45472
Quality Assurance:
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected glial fibrillary acidic protein on 10 μg of Mouse Brain lysates.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
APur
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Chicken
Purification Method:
Immunoaffinity Purified