Product Images
Western Blot Analysis:
NT2/D1 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-SOX2.
Proteins were vis...
Western Blotting Analysis: Representative lot data. Mouse embryonic stem cell lysate was probed with Anti-SOX2 (0.5 µg/mL). Proteins were visua...
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. Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates SOX2 (~37 kDa).'))
 and ReNcell VM Human Neural Progenitor Cells using Anti-Sox2 (Green). At a dilution of 1:500 staining appears as primarily nuclear.')){kind=spacer}
Anti-Sox2 Antibody | AB5603
Recommended Replacement for: AB5770| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Sox2 Antibody | AB5603
Replaces:
AB5770
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Transcription factor Sox2. On western blots of whole cell or nuclear extracts, the antibody recognizes a 34 kDa band corresponding to Sox2 that is not observed in cytosolic extracts.
Molecular Weight:
34 kDa
Immunogen:
Synthetic peptide corresponding to human Sox2 (Acc# P48431). The immunogen sequence is identical in human and mouse.
Background Information:
SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution.
Species Reactivity:
- Human
- Mouse
Application Notes:
Western blot: 0.5-1.0 μg/mL on mouse ES cell or human NT2/D1 cell extracts. Reacts with a band of ~34 kDa. At higher antibody concentrations, may also react with bands of ~45 kDa and ~52 kDa (unknown origin). The 34 kDa Sox2 band is not observed on western blots of cytosolic extracts.
Immunocytochemistry: 1:1,000 using a Cy3 conjugated secondary antibody (AP132C) on mouse ES cells.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:1,000 using a Cy3 conjugated secondary antibody (AP132C) on mouse ES cells.
Optimal working dilutions must be determined by the end user.
Control:
Immunoblot: Mouse or human embryonic stem cell lysate, mouse embryonic germ cell lysate
Immunocytochemistry: Human embryonic stem cells (H9 line)
Immunocytochemistry: Human embryonic stem cells (H9 line)
Purification Method:
Immunoaffinity purified
Presentation:
Affinity Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, and 0.05% Sodium Azide, pH7.4.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- SOX2
- ANOP3
- MGC2413
- MCOPS3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Western Blotting
Entrez Gene Summary:
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
UniProt Summary:
FUNCTION: SwissProt: P48431 # Can form ternary complexes with OCT-1 or OCT-3 but acts as a transcriptional activator of FGF-4 enhancer DNA sequences only in conjunction with OCT-3 (By similarity).
SIZE: 317 amino acids; 34310 Da
SUBCELLULAR LOCATION: Nucleus.
DISEASE: SwissProt: P48431 # Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. MCOPS3 is characterized by the association of microphthalmia or clinical anophthalmia with anomalies such as microcephaly, short stature, hypogonadotropic hypogonadism, esophageal atresia and neurologic manifestations.
SIMILARITY: SwissProt: P48431 ## Contains 1 HMG box DNA-binding domain.
SIZE: 317 amino acids; 34310 Da
SUBCELLULAR LOCATION: Nucleus.
DISEASE: SwissProt: P48431 # Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. MCOPS3 is characterized by the association of microphthalmia or clinical anophthalmia with anomalies such as microcephaly, short stature, hypogonadotropic hypogonadism, esophageal atresia and neurologic manifestations.
SIMILARITY: SwissProt: P48431 ## Contains 1 HMG box DNA-binding domain.
Brand Family:
Chemicon
Product Name:
Anti-Sox2 Antibody | AB5603
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit