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Rabbit anti-HES-1 (Catalog Number AB5702) immunofluorescence staining of paraformaldehyde-fixed differentiated human neural stem cells showing spotted...
Anti-HES-1
| 种属反应性 | 关键应用 | 宿主 | 形式 | 抗体类型 |
|---|---|---|---|---|
| H, M | IH, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt序列号:
UniProt Summary
FUNCTION: SwissProt: Q14469 # Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity).
SIZE: 280 amino acids; 29541 Da
SUBUNIT: Transcription repression requires formation of a complex with a corepressor protein (Groucho) such as TLE2 (By similarity). Interacts with SIRT1.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: ... 更多信息 »
SIZE: 280 amino acids; 29541 Da
SUBUNIT: Transcription repression requires formation of a complex with a corepressor protein (Groucho) such as TLE2 (By similarity). Interacts with SIRT1.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: ... 更多信息 »
GenBank序列号:
Entrez Gene Summary
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Description:
Anti-HES-1
Background Information:
Hes1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and Hes1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.
Alternate Names:
Hairy 1
Specificity:
Recognizes HES-1 (Hairy family), a Helix-loop-helix class of transcription factor.
Key Applications:
- Immunohistochemistry
- Western Blotting
Application Notes:
Western Blot:
1:200-1:1,000 dilution using ECL.
Immunohistochemistry:
1:200-1:1,000 dilution of a previous lot worked.
Optimal working dilutions must be determined by the end user.
1:200-1:1,000 dilution using ECL.
Immunohistochemistry:
1:200-1:1,000 dilution of a previous lot worked.
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Human and rodent. Reactivity with other species has not been confirmed.
Immunogen:
Synthetic peptide.
Molecular Weight:
29 kDa
Control:
Human neural stem cells, Raji cell lysate.
Presentation:
Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Storage Conditions:
Stable for 1 year at -80ºC from date of receipt.
Gene Symbol:
- HES1
- Hes1
- HL
- HHL
- FLJ20408
- HRY
- HES-1
Antibody Category:
Neuroscience
Antibody Sub-Category:
- Developmental Neuroscience
- Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
APur
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Rabbit
Purification Method:
Immunoaffinity Purified