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Ordering Information
- : AB5702
- : 100 μg
Product Images
Rabbit anti-HES-1 (Catalog Number AB5702) immunofluorescence staining of paraformaldehyde-fixed differentiated human neural stem cells showing spotted...
Anti-HES-1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IH, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-HES-1
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes HES-1 (Hairy family), a Helix-loop-helix class of transcription factor.
Molecular Weight:
29 kDa
Immunogen:
Synthetic peptide.
Background Information:
Hes1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and Hes1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.
Species Reactivity:
- Human
- Mouse
Species Reactivity Note:
Human and rodent. Reactivity with other species has not been confirmed.
Application Notes:
Western Blot:
1:200-1:1,000 dilution using ECL.
Immunohistochemistry:
1:200-1:1,000 dilution of a previous lot worked.
Optimal working dilutions must be determined by the end user.
1:200-1:1,000 dilution using ECL.
Immunohistochemistry:
1:200-1:1,000 dilution of a previous lot worked.
Optimal working dilutions must be determined by the end user.
Control:
Human neural stem cells, Raji cell lysate.
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Storage Conditions:
Stable for 1 year at -80ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- HES1
- Hes1
- HL
- HHL
- FLJ20408
- HRY
- HES-1
Alternate Names:
Hairy 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Key Applications:
- Immunohistochemistry
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q14469 # Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity).
SIZE: 280 amino acids; 29541 Da
SUBUNIT: Transcription repression requires formation of a complex with a corepressor protein (Groucho) such as TLE2 (By similarity). Interacts with SIRT1.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: Q14469 Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). & The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.
SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 Orange domain. ... hide » see more »
SIZE: 280 amino acids; 29541 Da
SUBUNIT: Transcription repression requires formation of a complex with a corepressor protein (Groucho) such as TLE2 (By similarity). Interacts with SIRT1.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: Q14469 Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). & The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins.
SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 Orange domain. ... hide » see more »
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-HES-1
Antibody Type:
Polyclonal Antibody