Ordering Information
- : AB5731
- : 100 µg
- : 1 mg/mL
Product Images
Western Blot Analysis:
Embryonic Mouse Brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Nanog (1:5...
Anti-Nanog Antibody, N-terminus | AB5731
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Nanog Antibody, N-terminus | AB5731
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Antibody recognizes Nanog.
Molecular Weight:
39 kDa (MW 34 kDa)
Epitope:
N-terminus
Immunogen:
Synthetic peptide from mouse Nanog.
Isotype:
IgG
Background Information:
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, mouse and rat.
Application Notes:
Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.
Control:
Whole cell lysate from mouse embryonic stem cells.
Quality Assurance:
Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates
Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.
Storage Conditions:
Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- FLJ12581
- FLJ40451
- hNanog
Alternate Names:
- Homeobox transcription factor Nanog
- Nanog homeobox
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
UniProt Summary:
Function: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation By similarity.
Subunit structure: Interacts with SMAD1 and SALL4 By similarity.
Subcellular location: Nucleus. Ref.10
Tissue specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Ref.1 Ref.2 Ref.3 Ref.5 Ref.8
Developmental stage: Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. Ref.10 Ref.3 Ref.8 Ref.6 Ref.9
Miscellaneous: Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.
Sequence similarities Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
Subunit structure: Interacts with SMAD1 and SALL4 By similarity.
Subcellular location: Nucleus. Ref.10
Tissue specificity: Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Ref.1 Ref.2 Ref.3 Ref.5 Ref.8
Developmental stage: Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. Ref.10 Ref.3 Ref.8 Ref.6 Ref.9
Miscellaneous: Exists an other tandem duplicated non-processed pseudogene (NANOGP1) and 10 other NANOG-related nucleotide sequences located on different chromosomes, all of which are processed pseudogenes lacking introns (NANOGP2 to NANOGP11); except NANOGP8 which is a retrogene.
Sequence similarities Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
Brand Family:
Chemicon
Product Name:
Anti-Nanog Antibody, N-terminus | AB5731
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Affinity Purified
Host:
Rabbit