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Ordering Information
- : AB5804
- : 50 µL
- : Variable
Product Images
Rabbit anti-GFAP (Catalog Number AB5804). Staining of cultured neonatal rat forebrain with anti-GFAP is shown in green, while neuronal processes were ...
Chicken anti-Vimentin (Catalog Number AB5733). Localization of Vimentin (green) and GFAP (red, AB5408) in rat cerebral cortex neurons. Note flattened ...
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Anti-Glial Fibrillary Acidic Protein (GFAP)
Recommended Replacement for: AB1540| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ca, H, R | IC, IH, IH(P) | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Glial Fibrillary Acidic Protein (GFAP)
Replaces:
AB1540
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Glial Fibrillary Acidic Protein (GFAP).
Molecular Weight:
50 kDa
Immunogen:
Purified bovine GFAP
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Species Reactivity:
- Bovine
- Canine
- Human
- Rat
Species Reactivity Note:
Human, rat, bovine and canine. It is expected that the antibody will react with most mammalian GFAP due to homology. Reactivity with other species has not been confirmed.
Application Notes:
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
Representative images from a previous lot.
Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
Control:
Mouse brain tissue, rat astrocytes, rat neurons.
Quality Assurance:
Routinely evaluated by immunohistochemistry on brain tissue, astrocytes, and neurons.
Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown).
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain
Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown).
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain
Purification Method:
Unpurified
Presentation:
Rabbit serum. Liquid. Contains no preservative.
Storage Conditions:
Stable for 1 year at -20°C undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- FLJ45472
- GFAP
Alternate Names:
glial fibrillary acidic protein
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Entrez Gene Summary:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
UniProt Summary:
FUNCTION: SwissProt: Q28115 # GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 428 amino acids; 49512 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
SIMILARITY: SwissProt: Q28115 ## Belongs to the intermediate filament family.
SIZE: 428 amino acids; 49512 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
SIMILARITY: SwissProt: Q28115 ## Belongs to the intermediate filament family.
Brand Family:
Chemicon
Product Name:
Anti-Glial Fibrillary Acidic Protein (GFAP)
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Serum
Host:
Rabbit