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Rabbit anti-GFAP (Catalog Number AB5804) and Chicken anti-NF-H (Catalog Number AB5539). Staining of cultured neonatal rat forebrain with the anti-GFAP...
Chicken anti-Neurofilament H (Catalog Number AB5539) localization of NF-H (green) in cultured neonatal rat forebrain cells. Red staining in GFAP (Cata...
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Anti-Glial Fibrillary Acidic Protein (GFAP)
Produit de remplacement recommandé pour: AB1540| Espèces réactives | Principales applications | Hôte | Forme | Type d'anticorps |
|---|---|---|---|---|
| B, Ca, H, R | IC, IH, IH(P) | Rabbit | Serum | Polyclonal Antibody |
Code UniProt:
UniProt Summary
FUNCTION: SwissProt: Q28115 # GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 428 amino acids; 49512 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
SIMILARITY: SwissProt: Q28115 ## Belongs to the intermediate filament family.
SIZE: 428 amino acids; 49512 Da
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
SIMILARITY: SwissProt: Q28115 ## Belongs to the intermediate filament family.
Code GenBank:
Entrez Gene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Description:
Anti-Glial Fibrillary Acidic Protein (GFAP)
Replaces:
AB1540
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Alternate Names:
glial fibrillary acidic protein
Specificity:
Recognizes Glial Fibrillary Acidic Protein (GFAP).
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
Representative images from a previous lot.
Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in Immunocytochemistry.
Species Reactivity:
- Bovine
- Canine
- Human
- Rat
Species Reactivity Note:
Human, rat, bovine and canine. It is expected that the antibody will react with most mammalian GFAP due to homology. Reactivity with other species has not been confirmed.
Immunogen:
Purified bovine GFAP
Molecular Weight:
50 kDa
Control:
Mouse brain tissue, rat astrocytes, rat neurons.
Presentation:
Rabbit serum. Liquid. Contains no preservative.
Storage Conditions:
Stable for 1 year at -20°C undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- FLJ45472
- GFAP
Quality Assurance:
Routinely evaluated by immunohistochemistry on brain tissue, astrocytes, and neurons.
Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown).
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain
Immunohistochemistry(paraffin) Analysis:
GFAP (IHC2078) staining of Human Brain. Tissue pretreated with Citrate Buffer, pH 6.0. Pre-diluted polyclonal antibody, using IHC-Select® Detection with HRP-DAB. Glial cells stain strongly (brown).
Optimal Staining with Citrate Buffer Epitope Retrieval: Human Brain
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Serum
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Format:
Serum
Host:
Rabbit
Purification Method:
Unpurified