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Ordering Information

  • : AB5922
  • : 50 µL
  • : Please refer to the Certificate of Analysis for the lot-specific concentration.

Product Images

Rabbit anti-Nestin (Catalog Number AB5922) Staining of U251 cells. Shown are cells stained with anti-Nestin (green) and bisbenzimide (blue). Photo co...

Immunocytochemistry: Representative image from a previous lot.
Rabbit anti-Nestin (Catalog Number AB5922) Staining of U251 cells. Shown are cells...

Anti-Nestin Antibody

Species Reactivity Key Applications Host Format Antibody Type
H, not M, not R ICC, IHC, IH(P), WB Rabbit Serum Polyclonal Antibody
Description:
Anti-Nestin Antibody
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Human Nestin. The antibody shows no reactivity to vimentin or GFAP.
Molecular Weight:
200-220 kDa
Immunogen:
Fusion protein.
Isotype:
Background Information:
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
Species Reactivity:
Human
Species Reactivity Note:
Human. No reactivity to rat or mouse nestin. Other species have not yet been tested
Non-Reactive Species:
  • Mouse
  • Rat
Application Notes:
Western Blot:
1:1,000-1:5,000 dilution of a previous lot was used on cultured cells or human brain tissue. It is suggested that the membrane be blocked with PBS containing 5% nonfat milk and 0.05% Tween. The antibody may also be diluted in the same blocking buffer.

Immunocytochemistry:
1:200 dilution of a previous lot was used on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton.

Immunohistochemistry(paraffin):
1:200 dilution of a previous lot was used on formalin fixed, paraffin embedded tissue sections.
Control:
  • Positive controls are U251 or U373 human glioma cell lines. Detects a ~200-220 kDa protein, note slightly difference sizes for nestin are quoted in various paper and from various cell lines
  • in WI-38 (human lung line) reduced nestin migrates at ~220 kDa
  • in U251 (Human Glioma line) nestin migrates at ~260 kDa.
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal Serum in buffer containing Liquid.
Storage Conditions:
Stable at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
  • FLJ21841
  • Nbla00170
  • OTTHUMP00000032198
  • nestin
Alternate Names:
Nestin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Entrez Gene Summary:
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P48681 # May play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division (By similarity).
SIZE: 1621 amino acids; 177439 Da
SUBUNIT: Interacts with FHOD3 (By similarity).
TISSUE SPECIFICITY: CNS stem cells.
DEVELOPMENTAL STAGE: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
SIMILARITY: SwissProt: P48681 ## Belongs to the intermediate filament family.
Brand Family:
Chemicon
Product Name:
Anti-Nestin Antibody
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Serum
Host:
Rabbit
Applications:
Anti-Nestin Antibody is an antibody against Nestin for use in IC, IH, IH(P) & WB.

Product Resources

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