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Ordering Information
- : AB5977
- : 100 μg
Anti-Musashi-1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IC, IH, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Musashi-1
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Musashi-1.
Molecular Weight:
39 kDa
Immunogen:
Synthetic peptide, amino acids 5-21 of Musashi.
Background Information:
In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi1 is a selective marker for intestinal stem or early lineage cells. Musashi1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi1 is present.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human and rodent. Reactivity with other species has not been confirmed.
Application Notes:
Immunohistochemistry:
1:200-1:1,000 dilution from a previous lot was used.
Immunocytochemistry:
1:200-1:1,000 dilution from a previous lot was used.
Western blot:
1:200-1:1,000 using ECL.
Optimal working dilutions must be determined by the end user.
1:200-1:1,000 dilution from a previous lot was used.
Immunocytochemistry:
1:200-1:1,000 dilution from a previous lot was used.
Western blot:
1:200-1:1,000 using ECL.
Optimal working dilutions must be determined by the end user.
Control:
Mouse cortical neural stem cells (Catalog No. SCR029).
Quality Assurance:
Routinely evaluated by Western Blot on Human Placenta lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Musashi-1 on 10 μg of Human placenta lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Musashi-1 on 10 μg of Human placenta lysates.
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Storage Conditions:
Stable for 1 year at -80ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MSI1
- Musashi-1
Alternate Names:
Musashi homolog 1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: O43347 # RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
SIZE: 362 amino acids; 39125 Da
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
TISSUE SPECIFICITY: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
DOMAIN: SwissProt: O43347 The first RNA recognition motif binds more strongly to RNA compared to the second one (By similarity).
SIMILARITY: Contains 2 RRM (RNA recognition motif) domains.
SIZE: 362 amino acids; 39125 Da
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
TISSUE SPECIFICITY: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
DOMAIN: SwissProt: O43347 The first RNA recognition motif binds more strongly to RNA compared to the second one (By similarity).
SIMILARITY: Contains 2 RRM (RNA recognition motif) domains.
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-Musashi-1
Antibody Type:
Polyclonal Antibody