Anti-TIMP-1, C-terminus

Anti-TIMP-1 Antibody, C-terminus | AB6007

AB8112

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The antibody recognizes TIMP-1 at the C-terminus.

The calculated molecular weight is ~ 23 kDa. A band, ~29 kDa, is observed in Western Blot (2 glycosylation sites add ~ 5 kDa each).

C-terminus

KLH-conjugated synthetic peptide corresponding to human TIMP-1 at and around the C-terminus.

Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-1 levels in quiescent cells and tissues are low (pg/ml), and stimulation with the phorbol ester TPA or protein concentration is often needed to visualize protein bands. Additionally, cell types greatly differ in the amount of TIMP-1 produced.

Human

Human.

Western Blot (SNAP ID) Analysis: 1:500 dilution of a previous lot detected TIMP-1 on 10 µg of PMA treated HL-60 cell lysate.

PMA-treated HL-60 cell lysate

Evaluated by Western blot in PMA-treated HL-60 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-1 on 10 µg of PMA-treated HL-60 cell lysate.

Antigen affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

P01033

NP_003245.1

• TIMP1
• TIMP
• CLGI
• EPA
• EPO
• TIMP-1
• HCI

• Collagenase inhibitor
• Fibroblast collagenase inhibitor
• TIMP metallopeptidase inhibitor 1
• Tissue inhibitor of metalloproteinase 1

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Western Blotting

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

FUNCTION: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

SUBCELLULAR LOCATION: Secreted.

PTM: The activity of TIMP1 is dependent on the presence of disulfide bonds.

SEQUENCE SIMILARITIES: Belongs to the protease inhibitor I35 (TIMP) family.

Contains 1 NTR domain.

Anti-TIMP-1, C-terminus

0.5 mg/mL

Polyclonal Antibody

100 μg

Affinity Purified

Rabbit