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Ordering Information
- : AB9246
- : 100 µL
Anti-Non-selenium Glutathione Peroxidase
Recommended Replacement for: AB3700| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | WB, IH | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Non-selenium Glutathione Peroxidase
Replaces:
AB3700
Trade Name:
Chemicon (Millipore)
Specificity:
Nonselenium glutathione peroxidase (NSGP, Clara cell protein 26 kDa, 1-Cys peroxiredoxin).
Immunogen:
Recombinant rat nonselenium glutathione peroxidase.
Species Reactivity:
- Human
- Rat
Application Notes:
Western blotting: 1:1000-1:4000
Immunohistochemistry on frozen or wax embedded tissue: 1:200-1:2,000. Antigen retrieval is not necessary but optimal working dilutions will need to be determined.
STAINING PATTERN: Stains cytoplasm of epithelial cells in the rat and mouse lung and gut and rat and human brain astrocytes. Stains human astrocytes in Parkinson's and Alzheimer's disease and the central core of some Lewy bodies in dementia with Lewy bodies and Parkinson's disease.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on frozen or wax embedded tissue: 1:200-1:2,000. Antigen retrieval is not necessary but optimal working dilutions will need to be determined.
STAINING PATTERN: Stains cytoplasm of epithelial cells in the rat and mouse lung and gut and rat and human brain astrocytes. Stains human astrocytes in Parkinson's and Alzheimer's disease and the central core of some Lewy bodies in dementia with Lewy bodies and Parkinson's disease.
Optimal working dilutions must be determined by the end user.
Presentation:
Rabbit serum. Liquid.
Storage Conditions:
Maintain material at -20°C under sterile conditions for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PRDX6
- p29
- 1-Cys
- Peroxiredoxin-6
- MGC46173
- KIAA0106
- PRX
- NSGPx
- aiPLA2
- AOP2
- EC 1.11.1.15
- EC 3.1.1.-
- EC 1.11.1.7
Alternate Names:
NSGP, Clara Cell Protein 26, 1-Cys Peroxiredoxin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Key Applications:
- Western Blotting
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P30041 # Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
SIZE: 224 amino acids; 25035 Da
SUBUNIT: Homotetramer. May interact with HTR2A (By similarity). Interacts with STH.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Lysosome (By similarity). Cytoplasmic vesicle (By similarity). Note=And also found in lung secretory organelles (By similarity).
SIMILARITY: SwissProt: P30041 ## Belongs to the ahpC/TSA family. Rehydrin subfamily.
MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin. & Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress.
SIZE: 224 amino acids; 25035 Da
SUBUNIT: Homotetramer. May interact with HTR2A (By similarity). Interacts with STH.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Lysosome (By similarity). Cytoplasmic vesicle (By similarity). Note=And also found in lung secretory organelles (By similarity).
SIMILARITY: SwissProt: P30041 ## Belongs to the ahpC/TSA family. Rehydrin subfamily.
MISCELLANEOUS: The active site is the redox-active Cys-47 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin. & Irreversibly inactivated by overoxidation of Cys-47 (to Cys-SO(3)H) upon oxidative stress.
Qty/Pk:
100 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Rabbit
Product Name:
Anti-Non-selenium Glutathione Peroxidase
Antibody Type:
Polyclonal Antibody