Ordering Information
- : AB941
- : 100 µL
Anti-S-100 Protein
Recommended Replacement for: CBL410| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | IH, IH(P) | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-S-100 Protein
Replaces:
CBL410
Trade Name:
Chemicon (Millipore)
Specificity:
The antiserum supplied has been evaluated for its ability to give a positive and specific immunostaining of S-100-containing cells present in normal skin and human neoplasms using both the immunoperoxidase and immunofluorescence procedures.
Immunogen:
Purified bovine S-100 bound to keyhole limpet hemocyanin (KLH) with carbodiimide.
Species Reactivity:
- Human
- Rat
Application Notes:
TISSUE EVALUATION:
Formalin-fixed and paraffin embedded sections (6 microns thick) of human skin and squamous cell carcinomas were prepared. Cryostat sections of the above tissues may also be used.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated with phosphate buffered saline (PBS), pH 7.4.
2. The antiserum diluted 1:250-1:500 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess reagents with PBS, the section is treated wit fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
COMMENTS:
The antiserum described here has been established to give a positive staining of the S-100 cells using the above protocols. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in the incubation time, e.g. 24 hours.
Formalin-fixed and paraffin embedded sections (6 microns thick) of human skin and squamous cell carcinomas were prepared. Cryostat sections of the above tissues may also be used.
IMMUNOPEROXIDASE
STAINING:
1. The sections to be stained are hydrated with phosphate buffered saline (PBS), pH 7.4.
2. The antiserum diluted 1:250-1:500 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.
3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.
4. The final stage of the reaction is treatment of the section with a mixture of 3,3'-diaminbenzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.
IMMUNOFLUORESCENCE
STAINING:
1. Same as Step 1 above.
2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.
3. After washing off the excess reagents with PBS, the section is treated wit fluorescein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
COMMENTS:
The antiserum described here has been established to give a positive staining of the S-100 cells using the above protocols. It is recommended, however, that the customer establishes his own optimal dilutions to give positive staining. In general, staining can occur on further dilution of the primary antiserum if this is accompanied by an increase in the incubation time, e.g. 24 hours.
Presentation:
Rabbit serum. Liquid. Contains 0.1% sodium azide.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- S100A1
- S100A
- S100
- S100-alpha
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.
Key Applications:
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P23297 # Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
SIZE: 94 amino acids; 10546 Da
SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
SIMILARITY: SwissProt: P23297 ## Belongs to the S-100 family. & Contains 2 EF-hand domains.
SIZE: 94 amino acids; 10546 Da
SUBUNIT: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
SIMILARITY: SwissProt: P23297 ## Belongs to the S-100 family. & Contains 2 EF-hand domains.
Qty/Pk:
100 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Rabbit
Protein/Isoform Description:
S100 protein is a dimer that can be composed of two α (S100A), two β (S100B) or one α and one β (S100AB) subunit. The precise function of S100 is not yet known, but S100 is a Ca2+-binding EF-hand protein and is thought to regulate cell growth and differenetiation, cytoskeletal dynamics and Ca2+ homeostasis. Although formerly thought to be brain-specific, S100 has been found in a wide range of tissues and transformed cells; presence of S100A in serum may provide a marker for cardiac infarction.
Product Name:
Anti-S-100 Protein
Antibody Type:
Polyclonal Antibody