Ordering Information

Catalogue Number : AB9750
Qty/Pk : 100 µL

Product Images

Western Blot of rat brainstem lysate. The immunolabeling is completely eliminated by blocking the antibody with the phosphor peptide.

Anti-14-3-3 phospho Serine58

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB	Rabbit	Affinity Purified	Polyclonal Antibody

Description:

Trade Name:

Chemicon (Millipore)

Specificity:

14-3-3 Protein, phosphoSerine58. The antibody recognizes a protein of ~29 kDa corresponding to 14-3-3 Protein, phosphoSerine58 in lysates from rat brainstem. Immunolabeling is blocked by the phosphopeptide used as the immunogen but not the corresponding non-phosphopeptide.

Immunogen:

Synthetic peptide of amino acids surrounding the phosphoSerine 58 site of rat 14-3-3 Protein.

Modifications:

Phosphorylation

Species Reactivity:

Human
Rat

Species Reactivity Note:

The immunogen sequence has 100% conservation with mouse and Xenopus.

Application Notes:

Western blot: 1:1,000

Purification Method:

Immunoaffinity purified

Presentation:

Liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Storage Conditions:

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

UniProt Number:

P62258

Entrez Gene Number:

NM_006761.3

Gene Symbol:

YWHAE
KCIP-1
MDS
14-3-3E
MDCR
FLJ45465

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Entrez Gene Summary:

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.

Key Applications:

Western Blotting

UniProt Summary:

FUNCTION: SwissProt: P62258 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
SIZE: 255 amino acids; 29174 Da
SUBUNIT: Homodimer. Interacts with NDEL1 (By similarity). Interacts with HCV core protein.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
SIMILARITY: SwissProt: P62258 ## Belongs to the 14-3-3 family.

Qty/Pk:

100 µL

Brand Family:

Chemicon

Format:

Affinity Purified

Host:

Rabbit

Protein/Isoform Description:

The 14-3-3 proteins are a family (at least seven members in mammals) of linkers and adaptors that comprise as much as 1% of total cellular protein. The proteins serve many functions, binding to other cytoplasmic and nuclear proteins through phosphorylated serine or threonine residues in virtually all eukaryotic cells. These binding interactions frequently have regulatory significance in many key cellular processes such as cell cycle progression and apoptosis, cell adhesion, and cell morphology. Aberrations in expression of 14-3-3 isoform expression has been linked to several genetic disorders and to cancer.

Product Name:

Anti-14-3-3 phospho Serine58

Antibody Type:

Polyclonal Antibody