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Número de catálogo : AB980
Qtde/cx : 500 µL
Conc. : Variable
Preço de Lista : RMB 6,108.57
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Anti-Myelin Basic Protein

Reatividade à Espécie	Aplicações Essenciais	Hospedeiro	Formato	Tipo de Anticorpo
H, M	IH, IH(P), WB	Rabbit	Purified	Polyclonal Antibody

Número Uniprot:

P02686

UniProt Summary

FUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
SIZE: 304 amino acids; 33117 Da
SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family. ... see more »

Número Genbank:

NM_001025094.1

Entrez Gene Summary

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related t ... see more »

Description:

Anti-Myelin Basic Protein

Background Information:

The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.

Alternate Names:

Myelin A1 protein
Myelin membrane encephalitogenic protein

Specificity:

Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.

Key Applications:

Immunohistochemistry
Immunohistochemistry (Paraffin)
Western Blotting

Application Notes:

Immunohistochemistry:
1:200 dilution of a previous lot was used by PAP or ABC on formalin fixed, paraffin-embedded human spinal cord. Pretreatment of tissue with proteolytic enzyme is not required.

Western Blot:
A dilution of 1:3000 was used in TTBS, O/N 4°C on a previous lot.

Optimal working dilutions must be determined by the end user.

Species Reactivity:

Human
Mouse

Immunogen:

Human myelin basic protein from brain

Molecular Weight:

21, 18, 17 & 14 kDa.

Control:

Brain tissue, brain lysate

Presentation:

Liquid in 0.05 mol/L Tris-HCl, pH 7.6 with 15 mmol/L sodium azide.

Storage Conditions:

Stable for 1 year at 2-8ºC from date of receipt.

Gene Symbol:

MBP
MGC99675
Golli-mbp

Quality Assurance:

Evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected four isoforms of MBP on 10 µg of Mouse brain lysates.

Antibody Category:

Neuroscience

Antibody Sub-Category:

Neuronal & Glial Markers
Neurochemistry & Neurotrophins

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Trade Name:

Chemicon (Millipore)

Format:

Purified

Host:

Rabbit

Format Code:

Spur

Concentration:

Variable

Antibody Type:

Polyclonal Antibody