Ordering Information
- : ABS204
- : 50 µL
Product Images
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of DCA-treated HEK293 cells using Anti-phospho-PDHE1-...
Immunoprecipitation Analysis:
Representative lot data.
A 1:50 dilution of this antibody immunoprecipitated phospho-PDHE1-A type I (Ser293)...
 (Red). Actin filaments have been labeled with AlexaFluor® 488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm.'))
 in 375 µg of HEK293 RIPA lysate. Arrow indicates PDHE1-A type I (~43 kDa). A non-specific band is observed at molecular weight of ~65 kDa.'))
 and DCA-treated (lane 2) HEK293 cell lysates were probed with Anti-phospho-PDHE1-A type I (Ser293) (1:10,000 dilution) . <br />Proteins were visulaized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. <br />Arrow indicates PDHE1-A type I (~43 kDa).'))
Anti-phospho-PDHE1-A type I (Ser293)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, B, Pm | WB, ICC, IP | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-phospho-PDHE1-A type I (Ser293) Antibody | ABS204
Promotional Text:
Specificity:
This antibody recognizes PDHE1-A type I phosphorylated at Ser293.
Molecular Weight:
~43 kDa observed
Epitope:
Phosphorylated Ser293
Immunogen:
KLH-conjugated linear peptide corresponding to human PDHE1-A type I phosphorylated at Ser293.
Modifications:
Phosphorylation
Background Information:
In many organisms, the pyruvate dehydrogenase (PDH) complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2 in the aerobic pathway. This complex also serves as a key regulator for cardiac substrate selection. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1 or PDHE1-A type I), dihydrolipoamide acetyltransferase (E2), and lipoamide dehydrogenase (E3). PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition.
Species Reactivity:
- Human
- Rat
- Mouse
- Bovine
- Primate
Species Reactivity Note:
Demonstrated to react with Human.
Predicted to react with Rat, Mouse, Bovine, Zebra Fish, and Primate based on 100% sequence homology.
Predicted to react with Rat, Mouse, Bovine, Zebra Fish, and Primate based on 100% sequence homology.
Application Notes:
Immunocytochemistry Analysis: A 1:500 dilution of this antibody from a representative lot detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cells.
Control:
Untreated and DCA-treated HEK293 cell lysates
Quality Assurance:
Evaluated by Western Blot in DCA untreated and treated HEK293 cell lysates.
Western Blot Analysis: A 1:10,000 dilution of this antibody detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cell lysates.
Western Blot Analysis: A 1:10,000 dilution of this antibody detected phospho-PDHE1-A type I (Ser293) in untreated and DCA-treated HEK293 cell lysates.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PDHA1
- PHE1A
Alternate Names:
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunoprecipitation
Entrez Gene Summary:
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Summary:
FUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
COFACTOR: Thiamine pyrophosphate.
ENZYME REGULATION: E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.
SUBUNIT STRUCTURE: Tetramer of 2 alpha and 2 beta subunits.
SUBCELLULAR LOCATION: Mitochondrion matrix.
TISSUE SPECIFICITY: Ubiquitous.
CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
COFACTOR: Thiamine pyrophosphate.
ENZYME REGULATION: E1 activity is regulated by phosphorylation (inactivation) and dephosphorylation (activation) of the alpha subunit.
SUBUNIT STRUCTURE: Tetramer of 2 alpha and 2 beta subunits.
SUBCELLULAR LOCATION: Mitochondrion matrix.
TISSUE SPECIFICITY: Ubiquitous.
Product Name:
Anti-phospho-PDHE1-A type I (Ser293)
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Affinity Purified
Host:
Rabbit