Ordering Information
- : ABS222
- : 100 μg
- : 1 mg/mL
Product Images
Western Blotting Analysis:
Representative lot data.
HEK293 lysate was probed with Anti-DEPTOR (1 µg/mL).
Proteins were visualized us...
Anti-DEPTOR
Recommended Replacement for: 09-463| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-DEPTOR
Replaces:
09-463
Specificity:
This antibody recognizes the DEP domain that contains mTOR-interacting protein DEPTOR.
Molecular Weight:
~46 kDa observed.
Epitope:
DEP domain-containing mTOR-interacting protein DEPTOR.
Immunogen:
KLH-conjugated linear peptide corresponding to the DEP domain-containing mTOR-interacting protein of human DEPTOR.
Background Information:
DEPTOR (DEPDC6, DEP domain-containing protein 6) was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR (Peterson, T.R., et al. (2009). Cell. 137(5):873-876).
Species Reactivity:
Human
Species Reactivity Note:
Demonstrated to react with Human.
Other homologies: Primate, Equine, Porcine, Canine, Rat, and Mouse based on 99%, 99%, 98%, 97%, and 96% homology, respectively.
Other homologies: Primate, Equine, Porcine, Canine, Rat, and Mouse based on 99%, 99%, 98%, 97%, and 96% homology, respectively.
Control:
HEK293 cell lyaste
Quality Assurance:
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected DEPTOR in HEK293 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected DEPTOR in HEK293 cell lysate.
Purification Method:
Affinity Purified
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- DEPTOR
- DEPDC6
Alternate Names:
- DEP domain-containing mTOR-interacting protein
- DEP domain-containing protein 6
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: Negative regulator of the mTORC1 and mTORC2 signaling pathways. Inhibits the kinase activity of both complexes.
SUBUNIT STRUCTUREInteracts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2.
SUBUNIT STRUCTUREInteracts (via PDZ domain) with MTOR; interacts with MTOR within both mammalian target of rapamycin complexes mTORC1 and mTORC2.
Protein/Isoform Description:
DEPTOR (DEPDC6, DEP domain-containing protein 6) was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. DEPTOR negatively regulates mTORC1 and mTORC2. It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival. Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR (Peterson, T.R., et al. (2009). Cell. 137(5):873-876).
Product Name:
Anti-DEPTOR
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit