Ordering Information
- : ABS45
- : 200 μg
- : 0.5 mg/mL
Product Images
Western Blotting by Snap ID analysis:NIH3T3 +/- Calyculin/Okadaic acid lysate was resolved by electrophoresis, transferred to PVDF membranes and probe...
Western Blotting analysis:
HEK293 +/- Calyculin/Okadaic acid lysate was resolved by electrophoresis, transferred to PVDF membranes and probed wit...
 (1:1,000) dilution. <br />Proteins were visualized using a Dnk anti-Rbt IgG:HRP conjugated to HRP and visualized using a chemiluminescence detection system.<br />Arrow indicates Phospho-MYPT1 (Thr696) (~130 kDa).'))
 (1:5,000) dilution. <br />Proteins were visualized using a Dnk anti-Rbt IgG:HRP conjugated to HRP and visualized using a chemiluminescence detection system. <br />Arrow indicates Phospho-MYPT1 (Thr696) (~130 kDa).'))
 and Calyculin/Okadaic acid treated NIH/3T3 cell lysate (lane 2) was resolved by electrophoresis, transferred to PVDF membranes and probed with anti-phospho-MYPT1 (Thr696) (1:1,000) dilution. Proteins were visualized using a Donkey anti-Rabbit IgG conjugated to HRP and visualized using a chemiluminescence detection system. <br />Arrow indicates Phospho-MYPT1 (Thr696) (~130 kDa).'))
Anti-phospho-MYPT1 (Thr696)
Recommended Replacement for: 07-251| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Pm, R, Ca, Xn, Eq, Ch | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-phospho-MYPT1 (Thr696) Antibody | ABS45
Replaces:
07-251
Promotional Text:
Specificity:
This antibody recognizes phosphorylated MYPT1 at Thr696.
Molecular Weight:
Expected at 115 kDa and observed at 130 kDa.
Epitope:
Phosphorylated Thr696
Immunogen:
KLH-conjugated linear peptide corresponding to human phosphorylated Thr696 of MYPT1.
Modifications:
Phosphorylation
Background Information:
Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity.
Species Reactivity:
- Human
- Mouse
- Primate
- Rat
- Canine
- Xenopus
- Horse
- Chicken
Species Reactivity Note:
Reacts with human and mouse. Predicted to react with primate, dog, rat, xenopus, horse, chicken, cow, monkey, and danio based on sequence homology.
Application Notes:
Western Blotting (SNAP ID)Analysis: 0.1 µg/mL from a previous lot detected MYPT1 on 10 µg of NIH/3T3 cell lysate.
Control:
Calyculin/Okadaic treated and untreated HEK293 cell lysates
Quality Assurance:
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody was used to detect MYPT1 phosphorylated at Thr696 on 10 µg of Calyculin/Okadaic treated and untreated HEK293 cell lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody was used to detect MYPT1 phosphorylated at Thr696 on 10 µg of Calyculin/Okadaic treated and untreated HEK293 cell lysate.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MBS
- MGC133042
- MYPT1
Alternate Names:
- Myosin phosphatase target subunit 1
- Myosin phosphatase-targeting subunit 1
- Protein phosphatase myosin-binding subunit
- myosin phosphatase, target subunit 1
- protein phosphatase 1, regulatory (inhibitor) subunit 12
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small
guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells.The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of
phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA,
phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells.The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of
phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA,
phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Summary:
FUNCTION: Regulates myosin phosphatase activity.
SUBUNIT STRUCTURE: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT By similarity. Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note: Along actomyosin filaments and stress fibers.
PTM: Phosphorylated by CIT (Rho-associated kinase) By similarity. Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Contains 6 ANK repeats.
SUBUNIT STRUCTURE: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT By similarity. Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note: Along actomyosin filaments and stress fibers.
PTM: Phosphorylated by CIT (Rho-associated kinase) By similarity. Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
SEQUENCE SIMILARITIES: Contains 6 ANK repeats.
Product Name:
Anti-phospho-MYPT1 (Thr696)
Concentration:
0.5 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
200 μg
Format:
Affinity Purified
Host:
Rabbit