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Goat anti-Mouse IgG, Alkaline Phosphatase conjugate
Recommended Replacement for: AP126A| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, WB | Goat | Alkaline Phosphatase | Polyclonal Antibody |
Description:
Goat anti-Mouse IgG, Alkaline Phosphatase conjugate
Replaces:
AP126A
Trade Name:
Chemicon (Millipore)
Product Overview:
Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Mouse IgG (H+L)
Specificity:
Specific for mouse IgG, heavy and light chain.
Background Information:
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Species Reactivity:
Mouse
Application Notes:
EIA and Western blots: 1:5,000-1:50,000.
Immunohistochemistry: 1:1,000-1:2,000.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:1,000-1:2,000.
Optimal working dilutions must be determined by end user.
Purification Method:
Immunoaffinity purified
Presentation:
Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with
15 mg/mL BSA and 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
15 mg/mL BSA and 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.
Storage Conditions:
this product is stable for several weeks at 2–8°C as an undiluted liquid. DO NOT FREEZE. For
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Western Blotting
Qty/Pk:
1 mL
Brand Family:
Chemicon
Format:
Alkaline Phosphatase
Host:
Goat
Product Name:
Goat anti-Mouse IgG, Alkaline Phosphatase conjugate
Antibody Type:
Polyclonal Antibody