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Goat anti-Mouse IgG, biotin-SP conjugate
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, WB | Goat | Biotin | Polyclonal Antibody |
Description:
Goat anti-Mouse IgG, biotin-SP conjugate
Trade Name:
Chemicon (Millipore)
Product Overview:
Affinity purified goat anti-mouse IgG, Biotin conjugated.
Background Information:
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Species Reactivity:
Mouse
Application Notes:
EIA: 1:50,000-1:100,000
Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000
Flow cytometry: 1:200-1:1,000
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Western blots using enzyme-conjugated streptavidin: 1:10,000-1:100,000.
Immunohistochemistry: 1:500-1:5,000
Flow cytometry: 1:200-1:1,000
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,000
Optimal working dilutions must be determined by end user.
Purification Method:
Immunoaffinity purified
Presentation:
PRESENTATION: Lyophilized. Buffer = 0.02 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.1% sodium azide.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label.
Storage Conditions:
Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Western Blotting
Qty/Pk:
2 mL
Brand Family:
Chemicon
Format:
Biotin
Host:
Goat
Product Name:
Goat anti-Mouse IgG, biotin-SP conjugate
Antibody Type:
Polyclonal Antibody