Goat anti-Rabbit IgG, Alkaline Phosphatase conjugate
Recommended Replacement for: AP158A| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Rb | ELISA, WB | Goat | Alkaline Phosphatase | Polyclonal Antibody |
Description:
Goat anti-Rabbit IgG, Alkaline Phosphatase conjugate
Replaces:
AP158A
Background Information:
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
Key Applications:
- ELISA
- Western Blotting
Application Notes:
ELISA: 1:5,000-1:50,000
Western Blot: 1:5,000-1:50,000
Optimal working dilutions must be determined by the end user.
Western Blot: 1:5,000-1:50,000
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Rabbit
Presentation:
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge or vortex this product if it is not completely clear after standing for 1-2 hours at room temperature.
Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge or vortex this product if it is not completely clear after standing for 1-2 hours at room temperature.
Storage Conditions:
Store lyophilized material at 2-8°C until opened. After reconstitution this product is stable for several weeks at 2-8°C as an undiluted liquid. DO NOT FREEZE. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at 2-8°C for up to 12 months. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after adding glycerol.
Antibody Category:
Secondary & Control Antibodies
Antibody Sub-Category:
Whole Immunoglobulin Secondary Antibodies
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Alkaline Phosphatase
Host:
Goat
Product Overview:
Goat anti Rabbit IgG affinity purified antibody, Alkaline Phosphatase conjugated.
Concentration:
0.6mg/mL
Antibody Type:
Polyclonal Antibody
Purification Method:
immunoaffinity purified

