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  • : APT169
  • : 100 assays
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Caspase 6 Colorimetric Activity Assay Kit, VEID

Description:
Caspase 6 Colorimetric Activity Assay Kit, VEID
Trade Name:
Chemicon (Millipore)
Qty/Pk:
100 assays
Product Overview:
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Mch2/Caspase-6 Colorimetric Protease Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VEID. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VEID-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Mch2 activity.

For Research Use Only; not for use in diagnostic procedures
Applications:
The Mch2/Caspase-6 Colorimetric Protease Assay Kit provides a simple & convenient means for assaying the activity of caspases that recognize the sequence VEID.
Key Applications:
Activity Assay
Species Reactivity:
Mammals
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms.
UniProt Summary:
FUNCTION: SwissProt: P55212 # Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
SIZE: 293 amino acids; 33310 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
SIMILARITY: SwissProt: P55212 ## Belongs to the peptidase C14 family.
Components:
  • Cell Lysis Buffer - 100 mL
  • 2X Reaction Buffer - 4 x 2 mL
  • DTT (1 M): - 400 μL
  • VEID-pNA Substrate (4 mM): 500 μL
  • Dilution Buffer: 100 mL
Brand Family:
Chemicon
UniProt Number:
Storage Conditions:
We recommend storing reagents at -20°C upon arrival. (Store Cell Lysis Buffer, 2X Reaction Buffer, and Dilution Buffer at 4°C after opening) upon arrival. The performance of this product is guaranteed for 6 months.
Detection Methods:
Chromogenic
Gene Symbol:
  • CASP6
  • CASP-6
  • MCH2
  • EC 3.4.22.59 [Contains: Caspase-6 subunit p18
  • Caspase-6 subunit p11].
Product Name:
Caspase 6 Colorimetric Activity Assay Kit, VEID
Entrez Gene Number:
Alternate Names:
MCH-2
Materials Required but Not Delivered:
· Microcentrifuge and 1.5 mL Microcentrifuge tubes

· 37°C water bath or incubator

· Spectrophotometer equipped with a 400 to 405-nm filter.

· 96 well plate