Ordering Information
- : CBL1300
- : 500 μg
- : 0.5 mg/mL
Anti-VCAM-1, clone M/K - 2
Recommended Replacement for: MAB2242| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IH, IP, FUNC | Rat | Purified | Monoclonal Antibody |
Description:
Anti-VCAM-1, clone M/K - 2
Replaces:
MAB2242
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.
Clone:
M/K - 2
Isotype:
IgG1κ
Species Reactivity:
Mouse
Application Notes:
Studies of cell-cell interaction
Cell adhesion assays
Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures
Immunoprecipitation of the VCAM-1 integrin complex
Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.
Optimal working dilutions must be determined by the end user.
Cell adhesion assays
Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures
Immunoprecipitation of the VCAM-1 integrin complex
Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.
Optimal working dilutions must be determined by the end user.
Control:
EOMA and BMS2 endothelial cell lysates
Purification Method:
Protein A purfied
Presentation:
The monoclonal is presented in phosphate buffered saline containing 10mM sodium azide.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- VCAM1
- INCAM-100
- MGC99561
- CD106
- DKFZp779G2333
- L1CAM
Alternate Names:
CD106
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunohistochemistry
- Immunoprecipitation
- Affects Function
Entrez Gene Summary:
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
UniProt Summary:
FUNCTION: SwissProt: P19320 # Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
SIZE: 739 amino acids; 81276 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
DOMAIN: SwissProt: P19320 Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
PTM: Sialoglycoprotein.
DISEASE:SwissProt: P19320 # May play an important role in the genesis of atherosclerosis and rheumatoid arthritis.
SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
SIZE: 739 amino acids; 81276 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
DOMAIN: SwissProt: P19320 Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
PTM: Sialoglycoprotein.
DISEASE:SwissProt: P19320 # May play an important role in the genesis of atherosclerosis and rheumatoid arthritis.
SIMILARITY: Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Brand Family:
Chemicon
Product Name:
Anti-VCAM-1, clone M/K - 2
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
500 μg
Format:
Purified
Host:
Rat