Ordering Information
- : CBL1358
- : 500 µg
- : 0.5 mg/mL
Anti-Endoglin, Extracellular, clone MJ7/18
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IP, WB, IH, not IH(P) | Rat | Purified | Monoclonal Antibody |
Description:
Anti-Endoglin, Extracellular, clone MJ7/18
Trade Name:
Chemicon (Millipore)
Specificity:
Specifically recognizes mouse CD105, known as endoglin.
Immunogen:
Inflamed mouse skin (Ge, AZ et al, 1994).
Clone:
MJ7/18
Isotype:
IgG2aκ
Background Information:
Endoglin is the regulatory component of the TGF-beta receptor complex. CD105 is a homodimer of 90 kDa subunits and is predominantly expressed on vascular endothelial cells. It is also found on pre-erythroblasts, macrophages and lymphoid and myeloid leukemic cells High levels of mouse endoglin mRNA have been detected in ovary, uterus, and NCTC-2071 fibroblasts, and to a lesser extent, in heart and muscle. In addition, stromal cells in the connective tissue of various organs are also positive for endoglin expression. CD105 has been reported to have functions in adhesion and embryonic angiogenesis, and that MJ7/18 is a pan endothelial marker (with minimal reactivity with early hematopoetic cells). Both mouse and human CD105 exhibit >70% sequence similarity in their cytoplasmic domains with type III transforming growth factor β (TGFβ) receptor.
Species Reactivity:
Mouse
Application Notes:
Flow Cytometry: 1μg/ 10(e6) cells in 100 μL total volume.
Immunohistochemical staining of fresh frozen or acetone fixed tissue specimens.1:10-1:50. Not recommended for formalin fixed paraffin tissues.
Immunoprecipitation: Use rabbit anti-rat secondary antibody followed by protein A, or anti-rat beads for recovery.
Western Blotting: 1:500-1:1000. Recognizes a 90 kDa band under reducing conditions. Membrane preparations are recommended. Mouse vascular endothelial cells recommended as a positive control.
Optimal working dilutions must be determined by the end user.
Immunohistochemical staining of fresh frozen or acetone fixed tissue specimens.1:10-1:50. Not recommended for formalin fixed paraffin tissues.
Immunoprecipitation: Use rabbit anti-rat secondary antibody followed by protein A, or anti-rat beads for recovery.
Western Blotting: 1:500-1:1000. Recognizes a 90 kDa band under reducing conditions. Membrane preparations are recommended. Mouse vascular endothelial cells recommended as a positive control.
Optimal working dilutions must be determined by the end user.
Applications Not Recommended:
Immunohistochemistry (Paraffin)
Control:
POSITIVE CONTROL: mouse vascular endothelial cells
Presentation:
Protein A purified immunoglobulin presented as a liquid in 100 mM borate buffered saline, containing no preservatives.
Storage Conditions:
Maintain at 2-8°C for up to 12 months from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ENG
- ORW
- CD105
- HHT1
- END
- FLJ41744
- ORW1
- endoglin
Alternate Names:
CD105; Ancillary TGF-beta Receptor
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunoprecipitation
- Western Blotting
- Immunohistochemistry
Entrez Gene Summary:
Endoglin is a homodimeric transmembrane glycoprotein highly expressed by endothelial cells. It is a component of the transforming growth factor beta receptor complex as it binds TGFB1 and TGFB3 with high affinity. Mutations in the endoglin gene produce hereditary hemorrhagic telangiectasia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P17813 # Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
SIZE: 658 amino acids; 70578 Da
SUBUNIT: Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGF-beta receptors I and/or II. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Endoglin is restricted to endothelial cells in all tissues except bone marrow.
DISEASE: SwissProt: P17813 # Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
SIZE: 658 amino acids; 70578 Da
SUBUNIT: Homodimer that forms an heteromeric complex with the signaling receptors for transforming growth factor-beta: TGF-beta receptors I and/or II. It is able to bind TGF-beta 1, and 3 efficiently and TGF-beta 2 less efficiently.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Endoglin is restricted to endothelial cells in all tissues except bone marrow.
DISEASE: SwissProt: P17813 # Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
Brand Family:
Chemicon
Product Name:
Anti-Endoglin, Extracellular, clone MJ7/18
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
500 µg
Format:
Purified
Host:
Rat