Anti-CD45, clone MRC OX-1
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | FC, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-CD45, clone MRC OX-1
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody recognises a monomorphic determinant of the rat leucocyte common antigen (LCA, CD45). The antigen recognised is a heavily glycosylated membrane gylcoprotein of M.W. 170kD on thymocytes but M.W. 170- 220kD on other leucocytes (2,3). It is a useful marker for leucocytes as it has not been detected on any other cell type.
FUSION PARTNER: Spleen cells from BALB/c mice were fused with the cells of the NS1 mouse myeloma cell line.
FUSION PARTNER: Spleen cells from BALB/c mice were fused with the cells of the NS1 mouse myeloma cell line.
Immunogen:
Rat thymocyte membrane glycoproteins
Clone:
MRC OX-1
Isotype:
IgG1
Species Reactivity:
Rat
Application Notes:
Suitable for flow cytometry
Suitable for use on frozen sections
Optimal working dilutions must be determined by the end user.
Suitable for use on frozen sections
Optimal working dilutions must be determined by the end user.
Presentation:
The monoclonal is presented as 0.5mg at 1mg/mL in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PTPRC
- L-CA
- LCA
- CD45
- LY5
- T200
- glycoprotein
- B220
- GP180
- EC 3.1.3.48
Alternate Names:
LCA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.
Key Applications:
- Flow Cytometry
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P08575 # Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
SIZE: 1304 amino acids; 147254 Da
SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
PTM: Heavily N- and O-glycosylated.
DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.
SIZE: 1304 amino acids; 147254 Da
SUBUNIT: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08575 The first PTPase domain interacts with SKAP1.
PTM: Heavily N- and O-glycosylated.
DISEASE: SwissProt: P08575 # Defects in PTPRC are a cause of autosomal recessive severe combined immunodeficiency T-cell-negative/B-cell- positive/NK cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. SCID refers to a genetically and clinically group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms, including Candida albicans, Pneumocystis carinii, and cytomegalovirus, among many others. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T- cell development. & Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. & Contains 2 fibronectin type-III domains. & Contains 2 tyrosine-protein phosphatase domains.
Qty/Pk:
500 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Protein/Isoform Description:
CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.
Product Name:
Anti-CD45, clone MRC OX-1
Antibody Type:
Monoclonal Antibody