Anti-H-CAM, clone F10-44-2, Phycoerythrin conjugated

Anti-H-CAM, clone F10-44-2, Phycoerythrin conjugated

MAB2135E

Chemicon (Millipore)

The CD44 antigen is a transmembranous glycoprotein of 80 kDa, which has undergone both extensive O-linked and N-linked glycosylation. This antibody is specific for epitope 1. The CD44 antigen is present on T lymphocytes, granulocytes, red blood cells, brain and epithelial cells. There is weak expression on platelets. FUSION PARTNER: NS1 myeloma cell line

Purified human T cells

F10-44-2

IgG2a

Human

Identification of CD44 positive cells by flow cytometry and immunocytochemistry

Studies involving T, B cell, monocyte and granulocyte binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites

Studies on HCAM (homing receptor) function

Optimal working dilutions must be determined by the end user.

The conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.

P16070

• NM_000610.3
• NM_001001389.1
• NM_001001390.1
• NM_001001391.1
• NM_001001392.1

• CD44
• LHR
• HUTCH-I
• CDW44
• CDw44
• ECMR-III
• Epican
• MGC10468
• CD44R
• MDU2
• PGP-1
• CSPG8
• Pgp1
• IN
• MUTCH-I
• MDU3
• MIC4
• MC56
• HCELL

CD44

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flow Cytometry

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

FUNCTION: SwissProt: P16070 # Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
SIZE: 742 amino acids; 81554 Da
SUBUNIT: Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: An epithelial isoform (CD44E) is expressed by cells of epithelium and highly expressed by carcinomas. An hematopoietic isoform (CD44H) is expressed by cells of mesodermal origin. Expression is repressed in neuroblastoma cells.
PTM: Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. & N-glycosylated. & O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s). & Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
SIMILARITY: SwissProt: P16070 ## Contains 1 Link domain.

Chemicon

Anti-H-CAM, clone F10-44-2, Phycoerythrin conjugated

Monoclonal Antibody

100 assays

Phycoerythrin

Mouse