Anti-CD9, clone MM2/57
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Rb | FC, IP, WB, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-CD9, clone MM2/57
Trade Name:
Chemicon (Millipore)
Specificity:
This antibody reacts with a 25 kDa molecular weight band on gels of non-reduced platelet membranes and immunoprecipitates a similar band from Iodine125-labeled platelets. The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals localized in the cytoplasm.
Immunogen:
Human platelet glycoprotein
Clone:
MM2/57
Isotype:
IgG2bκ
Species Reactivity:
- Human
- Mouse
- Rabbit
Application Notes:
Immunohistochemistry: Frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues
Platelet inhibition and aggregation studies
Optimal working dilutions must be determined by the end user. the end user.
Platelet inhibition and aggregation studies
Optimal working dilutions must be determined by the end user. the end user.
Purification Method:
Protein A purfied
Presentation:
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
1 year at 4°C from date of shipment
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CD9
- Tspan-29
- MRP-1
- MIC3
- p24
- Tetraspanin-29
- BA2
- BTCC-1
- 5H9
- TSPAN29
- GIG2
- DRAP-27
- P24
Alternate Names:
MRP-1; DRAP-27
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunoprecipitation
- Western Blotting
- Immunohistochemistry
Entrez Gene Summary:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance.
UniProt Summary:
FUNCTION: SwissProt: P21926 # Involved in platelet activation and aggregation. Regulates paranodal junction formation. Required for gamete fusion. Involved in cell adhesion, cell motility and tumor metastasis.
SIZE: 228 amino acids; 25416 Da
SUBUNIT: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed by a variety of hematopoietic and epithelial cells.
PTM: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. & Phosphorylated on tyrosine following B-cell activation.
SIMILARITY: SwissProt: P21926 ## Belongs to the tetraspanin (TM4SF) family.
SIZE: 228 amino acids; 25416 Da
SUBUNIT: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed by a variety of hematopoietic and epithelial cells.
PTM: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. & Phosphorylated on tyrosine following B-cell activation.
SIMILARITY: SwissProt: P21926 ## Belongs to the tetraspanin (TM4SF) family.
Brand Family:
Chemicon
Product Name:
Anti-CD9, clone MM2/57
Concentration:
0.1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse