Anti-Cytokeratin 10, clone LH-2
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Cytokeratin 10, clone LH-2
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody reacts specifically with human cytokeratin 10 found in suprabasal cells of stratified squamous epithelia.
Clone:
LH-2
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
Identification of cytokeratin expressing cells by indirect immmunoperoxidase staining of frozen sections
Western blotting
IHC - formalin fixed sections. Pretreatment with type XXIV protease (0.5mg/ml) produces best results.
Optimal working dilutions must be determined by the end user.
Western blotting
IHC - formalin fixed sections. Pretreatment with type XXIV protease (0.5mg/ml) produces best results.
Optimal working dilutions must be determined by the end user.
Presentation:
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- KRT10
- K10
- CK-10
- Keratin-10
- Cytokeratin-10
- KPP
- CK10
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
UniProt Summary:
SIZE: 593 amino acids; 59511 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-10 is generally associated with keratin-1.
TISSUE SPECIFICITY: Seen in all suprabasal cell layers including stratum corneum.
DISEASE: SwissProt: P13645 # Defects in KRT10 are a cause of epidermolytic hyperkeratosis (EHK) [MIM:113800]; also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. & Defects in KRT10 are a cause of annular epidermolytic ichthyosis (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. & Defects in KRT10 are a cause of epidermal nevus epidermolytic hyperkeratotic type [MIM:600648]. Epidermal nevi affect about 1 in 1,000 people. They appear at or shortly after birth as localized lines of epidermal thickening. The extent of skin involvement varies widely.
SIMILARITY: SwissProt: P13645 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-10 is generally associated with keratin-1.
TISSUE SPECIFICITY: Seen in all suprabasal cell layers including stratum corneum.
DISEASE: SwissProt: P13645 # Defects in KRT10 are a cause of epidermolytic hyperkeratosis (EHK) [MIM:113800]; also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. & Defects in KRT10 are a cause of annular epidermolytic ichthyosis (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens. & Defects in KRT10 are a cause of epidermal nevus epidermolytic hyperkeratotic type [MIM:600648]. Epidermal nevi affect about 1 in 1,000 people. They appear at or shortly after birth as localized lines of epidermal thickening. The extent of skin involvement varies widely.
SIMILARITY: SwissProt: P13645 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-Cytokeratin 10, clone LH-2
Antibody Type:
Monoclonal Antibody