Ordering Information
- : CBL197
- : 100 µg
Anti-Cytokeratin 14, clone LL002
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Cytokeratin 14, clone LL002
Trade Name:
Chemicon (Millipore)
Specificity:
Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.
FUSION PARTNER: NS1 myeloma cell line
FUSION PARTNER: NS1 myeloma cell line
Immunogen:
Thyroglobulin conjugated synthetic peptide of extreme carboxy terminus of human keratin 14 (last 15 residues)
Clone:
LL002
Isotype:
IgG3
Species Reactivity:
Human
Application Notes:
Suitable for staining formalin fixed wax sections or frozen tissues. In order to obtain 100% consistency in staining it is considered essential to either microwave treat fixed tissue sections or alternatively use the more recently recommended high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use with CBL 197 is neutral buffered formalin.
Western blotting
Optimal working dilutions must be determined by the end user.
Western blotting
Optimal working dilutions must be determined by the end user.
Presentation:
The monoclonal is presented at a concentration of 100 μg/mL in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- KRT14
- Cytokeratin-14
- NFJ
- CK-14
- CK14
- EBS3
- EBS4
- K14
- Keratin-14
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
UniProt Summary:
SIZE: 472 amino acids; 51622 Da
SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-14 associates with keratin-5. Interacts with TRADD.
TISSUE SPECIFICITY: Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
DISEASE: SwissProt: P02533 # Defects in KRT14 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 601001]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mucous membranes as a result of mild trauma. The incidence at birth of all types of the disease is about 1:50,000. Three major clinical subgroups are: simplex (EBS), junctional (EBJ), and dystrophic (EBD). EBS is by far the most prevalent (65%). Blistering occurs within the basal cell layer and is further divided into three subgroups. Junctional and dystrophic EB are characterized by blistering in the plane of the lamina lucida of the basement membrane zone and below the dermal- epidermal basement membrane at the level of the anchoring fibrils, respectively. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is the most severe of the simplex group and is characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is characterized by blistering in palmar and plantar areas of the skin. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is characterized by generalized blistering as the Dowling-Meara type; the phenotype however is less severe. & Defects in KRT14 are the cause of Naegeli-Franceschetti- Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects. & Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistant reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
SIMILARITY: SwissProt: P02533 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-14 associates with keratin-5. Interacts with TRADD.
TISSUE SPECIFICITY: Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
DISEASE: SwissProt: P02533 # Defects in KRT14 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 601001]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mucous membranes as a result of mild trauma. The incidence at birth of all types of the disease is about 1:50,000. Three major clinical subgroups are: simplex (EBS), junctional (EBJ), and dystrophic (EBD). EBS is by far the most prevalent (65%). Blistering occurs within the basal cell layer and is further divided into three subgroups. Junctional and dystrophic EB are characterized by blistering in the plane of the lamina lucida of the basement membrane zone and below the dermal- epidermal basement membrane at the level of the anchoring fibrils, respectively. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is the most severe of the simplex group and is characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is characterized by blistering in palmar and plantar areas of the skin. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is characterized by generalized blistering as the Dowling-Meara type; the phenotype however is less severe. & Defects in KRT14 are the cause of Naegeli-Franceschetti- Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects. & Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistant reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
SIMILARITY: SwissProt: P02533 ## Belongs to the intermediate filament family.
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-Cytokeratin 14, clone LL002
Antibody Type:
Monoclonal Antibody