Guide d'achat
Anti-Glial Fibrillary Acidic Protein, clone GF12-24
| Espèces réactives | Principales applications | Hôte | Forme | Type d'anticorps |
|---|---|---|---|---|
| B, H, M, R | WB, IH(P) | Mouse | Purified | Monoclonal Antibody |
Code UniProt:
UniProt Summary
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander diseas ... En voir davantage »
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander diseas ... En voir davantage »
Code GenBank:
Entrez Gene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Description:
Anti-Glial Fibrillary Acidic Protein, clone GF12-24
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Alternate Names:
GFAP
Clone:
GF12-24
Specificity:
This antibody reacts with a 50 kDa GFAP in human brain sections. No cross-reactivity with other intermediate filaments has been observed. GFAP is a specific marker for astroglial cells.
KNOWN SPECIES CROSS REACTIVITY: Recognizes bovine, rat and mouse
KNOWN SPECIES CROSS REACTIVITY: Recognizes bovine, rat and mouse
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
Application Notes:
Classification of brain tumours
Suitable for formalin fixed paraffin wax embedded tissue sections
Pre-natal diagnosis of neural tube defects
Western blotting
Optimal working dilutions must be determined by the end user.
Suitable for formalin fixed paraffin wax embedded tissue sections
Pre-natal diagnosis of neural tube defects
Western blotting
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Bovine
- Human
- Mouse
- Rat
Isotype:
IgG2a
Presentation:
The monoclonal is presented as 50μg lyophilysed material. Reconstitute with 1ml distilled water. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Gene Symbol:
- GFAP
- FLJ45472
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Mouse
Replaced By:
MAB3402
Antibody Type:
Monoclonal Antibody