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Ordering Information
- : CBL411
- : 50 µg
Anti-Glial Fibrillary Acidic Protein, clone GF12-24
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-Glial Fibrillary Acidic Protein, clone GF12-24
Trade Name:
Chemicon (Millipore)
Specificity:
This antibody reacts with a 50 kDa GFAP in human brain sections. No cross-reactivity with other intermediate filaments has been observed. GFAP is a specific marker for astroglial cells.
KNOWN SPECIES CROSS REACTIVITY: Recognizes bovine, rat and mouse
KNOWN SPECIES CROSS REACTIVITY: Recognizes bovine, rat and mouse
Clone:
GF12-24
Isotype:
IgG2a
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Species Reactivity:
- Bovine
- Human
- Mouse
- Rat
Application Notes:
Classification of brain tumours
Suitable for formalin fixed paraffin wax embedded tissue sections
Pre-natal diagnosis of neural tube defects
Western blotting
Optimal working dilutions must be determined by the end user.
Suitable for formalin fixed paraffin wax embedded tissue sections
Pre-natal diagnosis of neural tube defects
Western blotting
Optimal working dilutions must be determined by the end user.
Presentation:
The monoclonal is presented as 50μg lyophilysed material. Reconstitute with 1ml distilled water. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- GFAP
- FLJ45472
Alternate Names:
GFAP
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
Qty/Pk:
50 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Replaced By:
MAB3402
Product Name:
Anti-Glial Fibrillary Acidic Protein, clone GF12-24
Antibody Type:
Monoclonal Antibody