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Anti-N-Myc, clone IA8
Recommended Replacement for: CBL437| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-N-Myc, clone IA8
Replaces:
CBL437
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody reacts with a major peptide-blockable band at 62-64 kDa on western blots.
Immunogen:
Human N-myc sequence 334-343 + Cys.
Clone:
IA8
Isotype:
IgG2b
Species Reactivity:
Human
Application Notes:
Western blotting on detergent lysates from cells expressing N-myc.
Immunohistochemical studies of N-myc on frozen sections.
Optimal working dilutions must be determined by the end user.
Immunohistochemical studies of N-myc on frozen sections.
Optimal working dilutions must be determined by the end user.
Presentation:
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- MYCN
- NMYC
- N-Myc
- BHLHE37
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. [provided by RefSeq].
Key Applications:
- Western Blotting
- Immunohistochemistry
UniProt Summary:
FUNCTION: May function as a transcription factor.
SIZE: 464 amino acids; 49561 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an heterodimer with MAX. Interacts with KDM5A, KDM5B and HUWE1.
SUBCELLULAR LOCATION: Nucleus.
DEVELOPMENTAL STAGE: Expressed during fetal development.
DISEASE: Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of Feingold syndrome; also known as oculodigitoesophagoduodenal syndrome (ODED). Feingold syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence.
SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain.
SIZE: 464 amino acids; 49561 Da
SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as an heterodimer with MAX. Interacts with KDM5A, KDM5B and HUWE1.
SUBCELLULAR LOCATION: Nucleus.
DEVELOPMENTAL STAGE: Expressed during fetal development.
DISEASE: Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of Feingold syndrome; also known as oculodigitoesophagoduodenal syndrome (ODED). Feingold syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence.
SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-N-Myc, clone IA8
Antibody Type:
Monoclonal Antibody