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  • : CBL468
  • : 100 μg
  • : 0.1 mg/mL
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  • Product Family Information

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Frozen section of tonsils showing endothelial staining (Catalog Number CBL468).

Anti-PECAM-1, domains 3-6 of human PECAM-1, clone HC1/6

Species Reactivity Key Applications Host Format Antibody Type
H FC, IH, IH(P), IP Mouse Purified Monoclonal Antibody
Description:
Anti-PECAM-1, domains 3-6 of human PECAM-1, clone HC1/6
Trade Name:
Chemicon (Millipore)
Specificity:
Reacts with a 130-140 kDa transmembranous glycoprotein that is expressed widely amongst cells of the vascular compartment. CD31 is expressed on platelets, cultured endothelial cells, monocytes, normal tissue macrophages in tonsil, lung and kidney as well as in skin biopsies from conditions such as sarcoidosis and lepromatous leprosy.
FUSION PARTNER: P3-x63Ag8.653 myeloma cell line
Epitope:
domains 3-6 of human PECAM-1
Immunogen:
PMA - treated U937 cells
Clone:
HC1/6
Isotype:
IgG1
Species Reactivity:
Human
Application Notes:
Studies of the role of CD31 in leucocyte migration into inflammatory sites and in the process of angiogenesis

This antibody will stain frozen tissue sections

Suitable for immunoprecipitation studies

Suitable for flow cytometry either indirectly or directly when labelled with FITC or R-PE

Suitable for use on formalin fixed paraffin embedded sections after protease pre-treatment.

Optimal working dilutions must be determined by the end user.
Control:
Activated microglial cells
Purification Method:
Protein A purfied
Presentation:
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
UniProt Number:
P16284
Entrez Gene Number:
NM_000442.3
Gene Symbol:
  • PECAM1
  • EndoCAM
  • GPIIA'
  • PECAM-1
  • CD31
  • CD31/EndoCAM
Alternate Names:
CD31
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
UniProt Summary:
FUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.
PTM: Phosphorylated on Ser and Tyr residues after cellular activation.
SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Brand Family:
Chemicon
Product Name:
Anti-PECAM-1, domains 3-6 of human PECAM-1, clone HC1/6
Concentration:
0.1 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 μg
Format:
Purified
Host:
Mouse

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