Ordering Information
- : CBL496
- : 100 µg
Product Images
Log fluorescence intensity profile of KG-1 cell line stained with Catalog Number CBL496.
Anti-CD34 Class II Antibody (CBL496) | Millipore
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Mk | FC, IP, IH, IH(P) | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-CD34 Class II Antibody (CBL496) | Millipore
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody recognizes a heavily glycosylated transmembrane protein: gp 105-120 kDa. The antigen is expressed on immature human haemopoietic precursor cells, capillary endothelial cells and human myeloid cells in the following categories: subsets of CFU-GEMM and BFU-E, all CFU-GM, granulocyte precursors, monocytes, myeloid leukaemias. This antibody recognizes the class II epitope showing resistance to neuraminidase enzyme cleavage.
KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry.
FUSION PARTNER: NSO myeloma cell line
KNOWN SPECIES CROSS REACTIVITY: Positive with rhesus monkey peripheral blood leucocytes in flow cytometry.
FUSION PARTNER: NSO myeloma cell line
Immunogen:
Human endothelial vesicles
Clone:
QBEND/10
Isotype:
IgG1
Species Reactivity:
- Human
- Monkey
Application Notes:
Identification of haemopoietic progenitor cells
Studies into the control mechanisms of early haemopoiesis
Purification of haemopoietic cells from, for example, FACS
The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts
Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0.
Optimal working dilutions must be determined by the end user.
Studies into the control mechanisms of early haemopoiesis
Purification of haemopoietic cells from, for example, FACS
The antibody reacts with both frozen and fixed tissue sections from vascular endothelium and stromal fibroblasts
Immunohistochemistry in parraffin sections: Pretreatment is HIER with citrate buffer, pH 6.0.
Optimal working dilutions must be determined by the end user.
Presentation:
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
CD34
Sales Restrictions:
This product is not available for sale in the United States.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunoprecipitation
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Entrez Gene Summary:
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM]
UniProt Summary:
FUNCTION: SwissProt: P28906 # Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
SIZE: 385 amino acids; 40716 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC.
DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis.
SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family.
SIZE: 385 amino acids; 40716 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
DEVELOPMENTAL STAGE: On early hematopoietic progenitor cells.
PTM: Highly glycosylated. & Phosphorylated on serine residues by PKC.
DISEASE:SwissProt: P28906 # Abnormal CD34 expression in leukemogenesis.
SIMILARITY: SwissProt: P28906 ## Belongs to the CD34 family.
Brand Family:
Chemicon
Product Name:
Anti-CD34 Class II Antibody (CBL496) | Millipore
Antibody Type:
Monoclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Mouse