Ordering Information
- : CBL541
- : 100 µg
Anti-CD16, clone GRM1
Recommended Replacement for: CBL525| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IF, IP, IH | Mouse | Purified | Monoclonal Antibody |
Description:
Anti-CD16, clone GRM1
Replaces:
CBL525
Trade Name:
Chemicon (Millipore)
Specificity:
A monoclonal antibody, GRM1, against a prolymphocytic leukemia that defines an antigen present in neutrophilic granulocytes (PMN) and a lymphocyte subset with natural killer (NK) activity, which was identified as large granular lymphocytes. This monoclonal antibody recognizes FcR2 (CD16), an antigen composed of two polypeptides of 50 and 60 kilodaltons, respectively. This GRM1 monoclonal antibody was tested against normal T and B cells, neutrophilic granulocytes, monocytes, platelets, acute and chronic leukemias, and was positive only against granulocytes (95%) and cells with NK activity. GRM1 was able to deplete NK cell activity in complement-dependent lysis. However, GRM1 did not block NK activity nor peripheral blood lymphocyte- and PMN-mediated antibody-dependent cytotoxicity in healthy individuals. GRM1 also did not block Fc receptor in an erythrocyte antibody rosette assay. The immunochemical data and cell distribution patterns lead us to conclude that GRM1 recognizes and FcR2 receptor epitope which is not involved in the receptor's function. Ruiz-Cabello, F et al (1987) "A study of GRM1 monoclonal antibody that reacts with natural killer cells and granulocytes." Nat Immun Cell Growth Requl 6(2):99-108.
Epitope:
Epitope has been mapped on the first, membrane-distal domain of CD16. {Tamm, A Schmidt, RE (1996) "The binding epitopes of human CD16 (Fc gamma RIII) monoclonal antibodies. Implications for ligand binding" J. Immunology 157(4):1576-1581}.
Clone:
GRM1
Isotype:
IgG2a
Background Information:
The CD16 molecule has been described as the low affinity Fc receptor (FcRIII) for complexed IgG which may exist either as a transmembranous form or as glycosyl phosphatidylinositol form. It is expressed on NK cells, granulocytes (PMN) and macrophages. The GPI-linked form is also expressed on neutrophils.
Antigen distribution:
Granulocytes > 98%
LGL cells > 85%
Peripheral blood lymphocytes 12+ 8%
Thymocytes < 1%
Monocytes < 1%
B cells (CD20+) < 1%
T cells (CD3+) < 1%
Antigen distribution:
Granulocytes > 98%
LGL cells > 85%
Peripheral blood lymphocytes 12+ 8%
Thymocytes < 1%
Monocytes < 1%
B cells (CD20+) < 1%
T cells (CD3+) < 1%
Species Reactivity:
Human
Application Notes:
Immunofluoresence: Identification of CD16+ leucocytes by indirect immunofluorescence staining of viable cell suspensions (unfixed).
Immunohistochemistry: (unfixed) Identification of CD16+ leucocytes present on fresh frozen tissue sections.
Studies on NK characterization and chronic myeloid leukaemia {Fernandez-Segura, E et al in Microscopy Research and Technique 28(4) pages 277-285. Wiley-Liss Inc publishers}. Ruiz-Cabello, F et al (1987) A study of GRM1 monoclonal antibody that reacts with Natural Killer Cells and Granulocytes {original paper}" Nat. Immun Cell Growth Regul. 6:99-108. Immunoprecipitation: GRM12 precipitated two polypeptides whose molecular weights were 53 and 60 kilodaltons from neutrophilic granulocytes.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: (unfixed) Identification of CD16+ leucocytes present on fresh frozen tissue sections.
Studies on NK characterization and chronic myeloid leukaemia {Fernandez-Segura, E et al in Microscopy Research and Technique 28(4) pages 277-285. Wiley-Liss Inc publishers}. Ruiz-Cabello, F et al (1987) A study of GRM1 monoclonal antibody that reacts with Natural Killer Cells and Granulocytes {original paper}" Nat. Immun Cell Growth Regul. 6:99-108. Immunoprecipitation: GRM12 precipitated two polypeptides whose molecular weights were 53 and 60 kilodaltons from neutrophilic granulocytes.
Optimal working dilutions must be determined by the end user.
Presentation:
The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- FCGR3A
- CD16a
- FcR-10
- FcRIIIa
- FcgammaRIIIA
- FcRIII
- FCG3
- CD16A
- CD16
- FCGR3
- IGFR3
Alternate Names:
FcgammaRIII
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunofluorescence
- Immunoprecipitation
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P08637 # Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
SIZE: 254 amino acids; 29089 Da
SUBUNIT: Exists as a hetero-oligomeric receptor complex with Fc epsilon receptor I gamma subunit and / or the CD3 zeta subunit. Interacts with INPP5D/SHIP1 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (Potential). Secreted. Note=Exists also as a soluble receptor.
TISSUE SPECIFICITY: Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
PTM: Glycosylated. Contains high mannose- and complex-type oligosaccharides. & The soluble form is produced by a proteolytic cleavage.
SIMILARITY: SwissProt: P08637 ## Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
MISCELLANEOUS: Encoded by one of two nearly indentical genes: FCGR3A and FCGR3B which are expressed in a tissue- specific manner. The Phe-203 in III-A determines the transmembrane domains whereas the Ser-203 in III-B determines the GPI-anchoring.
SIZE: 254 amino acids; 29089 Da
SUBUNIT: Exists as a hetero-oligomeric receptor complex with Fc epsilon receptor I gamma subunit and / or the CD3 zeta subunit. Interacts with INPP5D/SHIP1 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (Potential). Secreted. Note=Exists also as a soluble receptor.
TISSUE SPECIFICITY: Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
PTM: Glycosylated. Contains high mannose- and complex-type oligosaccharides. & The soluble form is produced by a proteolytic cleavage.
SIMILARITY: SwissProt: P08637 ## Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
MISCELLANEOUS: Encoded by one of two nearly indentical genes: FCGR3A and FCGR3B which are expressed in a tissue- specific manner. The Phe-203 in III-A determines the transmembrane domains whereas the Ser-203 in III-B determines the GPI-anchoring.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Mouse
Product Name:
Anti-CD16, clone GRM1
Antibody Type:
Monoclonal Antibody