Anti-Integrin αIIb Subunit, clone PM6/248, FITC conjugated

Anti-Integrin αIIb Subunit, clone PM6/248, FITC conjugated

Chemicon (Millipore)

The antibody recognizes the human CD41 cell surface antigen, a 140kDa glycoprotein expressed by platelets and megakaryocytes. CD41 is also known as platelet glycoprotein IIb, and functions as alpha receptor for fibrinogen, fibronectin and Von Willebrand Factor. It has not been established if clone PM6/248 recognises free CD41 or CD41 only when complexed with CD61. However, antibody binding is reduced in the presence of EDTA suggesting that the epitope recognized is dependent upon an intact CD41/61 complex.

FUSION PARTNER: SP2/0 myeloma cell line

PM6/248

IgG1

• Human
• Baboon

Identification of the alpha-integrin chain of gpIIb/IIa expression on platelets, megakaryocytes and monocytes by indirect immunofluorescence staining

Acute leukaemia phenotyping

Studies of Glanzmann's thrombasthenia where gpIIb/IIIa is absent or significantly reduced

Ligand binding occurs in part via an RGD sequence which is known to be of importance for certain other adhesion interactions with extracellular proteins

Optimal working dilutions must be determined by the end user.

The monoclonal is presented at a concentration of 100 tests/1 mL in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.

P08514

NM_000419.3

• ITGA2B
• CD41B
• HPA3
• GP2B
• CD41
• GPIIb
• ITGAB
• GTA

CD41

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

• Flow Cytometry
• Immunofluorescence

FUNCTION: SwissProt: P08514 # Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
SIZE: 1039 amino acids; 113391 Da
SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
DISEASE: SwissProt: P08514 # Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. This autosomal recessive disorder is the most common inherited disease of platelets. GT is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
SIMILARITY: SwissProt: P08514 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.

100 assays

Chemicon

FITC

Mouse

Anti-Integrin αIIb Subunit, clone PM6/248, FITC conjugated

Monoclonal Antibody