Human Collagen Type III

Human Collagen Type III

Chemicon (Millipore)

100 µg

Human type III collagen purified by serial salt precipitations, alcohol precipitation and DEAE chromatography of an acetic acid extraction of human placenta, following mild pepsin digestion. Composition: [a1(III)]3, native triple helix. Ability to form native helical structure verified by ORD measurement, competence in microfibril formation and reactivity with anti-collagen type-specific monoclonal antibodies

COL3A1 is the gene responsible for producing the pro-alpha1(III) chain of type III procollagen. Type III collagen, which adds structure and strength to connective tissues, is found in many places in the body, especially skin, lung, intestinal walls, and the walls of blood vessels. Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. Other enzymes add sugars to the protein. The type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen's tissue strengthening function.{http://ghr.nlm.nih.gov}

Cell Culture

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This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

FUNCTION: SwissProt: P02461 # Collagen type III occurs in most soft connective tissues along with type I collagen.
SIZE: 1466 amino acids; 138564 Da
SUBUNIT: Trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
DISEASE: SwissProt: P02461 # Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type III (EDS-III) [MIM:130020]; also known as benign hypermobility syndrome. Inheritance is autosomal dominant. EDS is characterized by joint laxity and hyperextensible skin. It is divided into nine different subtypes based on biochemical and clinical variations. & Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type IV (EDS-IV) [MIM:130050]. EDS-IV is the most severe form of the disease, in that it often produces life-threatening consequences, such as rupture of the arteries, bowel, or uterus. A variant form of EDS-IV is Gottron type acrogeria [MIM:201200]. The main characteristics are atrophy and mottled-type hyperpigmentation of the acral skin, resulting in an aged appearance. & Defects in COL3A1 may be a cause of aortic aneurysm [MIM:100070]. Aortic aneurysm consists of a dangerous ballooning of the aorta which is caused by a defect in the artery's wall.
SIMILARITY: SwissProt: P02461 ## Belongs to the fibrillar collagen family. & Contains 1 VWFC domain.

Human

• Human Embryonic Stem Cells
• Mesenchymal Stem Cells
• Neural Stem Cells
• Hematopoietic Stem Cells
• Epithelial Cells
• Pancreatic Stem Cells
• Induced Pluripotent Stem Cells

• Maintenance & Expansion
• General Cell Culture
• Differentiation

Chemicon

Human placenta, negative for HbsAG, HCV and HIV 1 & 2 antibodies.

Liquid in 0.5 M acetic acid, pH 2.5.

P02461

Maintain at -20ºC in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

• Stem Cell Reagents
• Attachment Factors

• COL3A1
• EDS4A
• FLJ34534

Extracellular Matrix Proteins

Human Collagen Type III

NM_000090.3

alpha 1 Type III Collagen

1 mg/mL

Human collagen type III: 90% pure by SDS-PAGE.

Human collagen type I: <10%

Human collagen types II, IV-VI, and non-collagen proteins: <1%

Retention of native structure was confirmed by ability to form microfibrils.