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Human Collagen Type V
Description:
Human Collagen Type V
Trade Name:
Chemicon (Millipore)
Qty/Pk:
100 µg
Product Overview:
PROPERTIES:
Molecular composition: [alpha1(V)]2 alpha2(V), native triple helix.
Purity and retention of native helical structure was monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies.
Molecular composition: [alpha1(V)]2 alpha2(V), native triple helix.
Purity and retention of native helical structure was monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies.
Key Applications:
Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II.
UniProt Summary:
FUNCTION: SwissProt: P20908 # Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
SIZE: 1838 amino acids; 183560 Da
SUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & Sulfated on 40% of tyrosines.
DISEASE: SwissProt: P20908 # Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type I (EDS-I) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS-I is a connective-tissue disorder characterized by loose-jointedness and fragile, velvety, stretchable, bruisable skin that heals with peculiar 'cigarette-paper' scars. Inheritance is autosomal dominant. & Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type II (EDS-II) [MIM:130010]; also known as Ehlers-Danlos syndrome mitis. Inheritance is autosomal dominant.
SIMILARITY: SwissProt: P20908 ## Belongs to the fibrillar collagen family. & Contains 1 laminin G-like domain. & Contains 1 TSP N-terminal (TSPN) domain.
SIZE: 1838 amino acids; 183560 Da
SUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & Sulfated on 40% of tyrosines.
DISEASE: SwissProt: P20908 # Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type I (EDS-I) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS-I is a connective-tissue disorder characterized by loose-jointedness and fragile, velvety, stretchable, bruisable skin that heals with peculiar 'cigarette-paper' scars. Inheritance is autosomal dominant. & Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type II (EDS-II) [MIM:130010]; also known as Ehlers-Danlos syndrome mitis. Inheritance is autosomal dominant.
SIMILARITY: SwissProt: P20908 ## Belongs to the fibrillar collagen family. & Contains 1 laminin G-like domain. & Contains 1 TSP N-terminal (TSPN) domain.
Species:
Human
Stem Cell Type:
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
- Maintenance & Expansion
- General Cell Culture
- Differentiation
Brand Family:
Chemicon
Source:
Human placenta, negative for HBsAG and HIV antibodies.
Presentation:
Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added.
UniProt Number:
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze.
Cell Culture Reagent Type:
- Stem Cell Reagents
- Attachment Factors
Gene Symbol:
- COL5A1
- OTTHUMP00000064637
Protein or Enzyme Type:
Extracellular Matrix Proteins
Product Name:
Human Collagen Type V
Entrez Gene Number:
Concentration:
1 mg/ml
Purity:
95% by SDS-PAGE
Contaminants: <2% collagen type I, <1% collagen type III, <2% collagen type IV and <0.5% non-collagen proteins.
PURIFICATION:
Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.
Contaminants: <2% collagen type I, <1% collagen type III, <2% collagen type IV and <0.5% non-collagen proteins.
PURIFICATION:
Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.