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Ordering Information
- : CC078
- : 10 mg
- : Starting concentration is recommended at 1 mg/mL
Collagen Type III, bovine purified protein
Description:
Collagen Type III, bovine purified protein
Trade Name:
Chemicon (Millipore)
Qty/Pk:
10 mg
Product Overview:
Molecular composition: a native triple helix, alpha1(III). Dissolved collagen retains the ability to form microfibrils, activate platelet aggregation and serve as a substrate for cell growth in culture.
Key Applications:
Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Bovine
Stem Cell Type:
- Mouse Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
- Maintenance & Expansion
- General Cell Culture
- Differentiation
Brand Family:
Chemicon
Source:
Bovine skin. Collagen is extracted from washed dissected tissue into dilute acetic acid after mild pepsin treatment.
Presentation:
Lyophilized protein containing no preservatives.
Recommended reconstitution instructions.
Add lyophilized collagen to 0.5 M acetic acid (3% glacial acetic acid v/v in water), 0.8 mL of solution to each mg of collagen.
Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension.)
Add acetic acid solution to dissolved collagen until solution is 1 mg/ml collagen. For large volumes, use a volumetric flask.
Recommended reconstitution instructions.
Add lyophilized collagen to 0.5 M acetic acid (3% glacial acetic acid v/v in water), 0.8 mL of solution to each mg of collagen.
Suspension should be shaken or stirred for several hours or overnight between 2-8°C. Material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction due to differing protein concentrations in the suspension.)
Add acetic acid solution to dissolved collagen until solution is 1 mg/ml collagen. For large volumes, use a volumetric flask.
UniProt Number:
Storage Conditions:
Maintain lyophilized material at -20° C up to one year from date of receipt. Upon reconstitution, maintain at -20° C in undiluted aliquots. Avoid repeated freeze/thaw cycles.
Cell Culture Reagent Type:
Attachment Factors
Protein or Enzyme Type:
Extracellular Matrix Proteins
Product Name:
Collagen Type III, bovine purified protein
Concentration:
Starting concentration is recommended at 1 mg/mL
Purity:
90% by reduced SDS-PAGE. Contains 10% bovine collagen type I and < 0.5% non-collagen proteins. Structure of native collagen confirmed by ability to form microfibrils.
Purification Method:
Differential salt precipitation.