Human Merosin

Human Merosin

Chemicon (Millipore)

500 µg

Human merosin protein affinity purified with monoclonal 5H2 (MAB1922). Clone 5H2binds the laminin alpha2 chain. In native purifications laminin 2 (alpha2beta1gamma1) and laminin 4 (alpha2beta2gamma1) isoforms are isolated via affinity purification over 5H2.
CC085 is immunologically and biologically similar to human laminin (Merosin is an oldername). This preparation contains several bands by SDS-PAGE, with primary bands at ~300-250 kDa, 200 and 80 kDa in 6% reducing gels.

Laminins are a family of large trimeric basement membrane glyco-proteins composed of 3 non-identical chains, the alpha-, beta- and gamma-chains. The three chains associate at their C-terminal end in a coiled coil. They form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. The long arm is formed by the coiled coil and the short arms by the N-terminal ends. Each laminin chain is a multi-domain protein encoded by a distinct gene. Several isoforms of each chain have been described. In human, five different alpha, three beta and two gamma chains have been identified, their genes dispersed throughout the genome. The biological functions of the different chains and trimer molecules are largely unknown. Some of the chains have been shown to differ extensively with respect to their tissue distribution, presumably reflecting diverse functions in vivo. The different laminin isoforms consist of different combinations of the alpha-, beta- and gamma-chains which are designated by Arabic numerals in the order of their discovery. Laminin-1 (alpha-1 + beta-1 + gamma-1), laminin-2 (merosin, alpha-2 + beta-1 + gamma-1), laminin-3 (alpha-1 + beta-2 + gamma-1), laminin-4 (alpha-2 + beta-2 + gamma-1), laminin-5 (alpha-3 + beta-3 + gamma-2), laminin-6 (alpha-3 + beta-1 + gamma-1), laminin-7 (alpha-3 + beta-2 + gamma-1), laminin-8 (alpha-4 + beta-1 + gamma-1), laminin-9 (alpha-4 + beta-2 + gamma-1) and laminin-10 (alpha-5 + beta-1 + gamma-1) (Miner et al., J Biol Chem 270(48):28523-28528, 1995). Laminin-2 and -4 are specifically enriched in the basement membranes surrounding skeletal muscle fibers and peripheral nerves. While the beta-1, beta-2 and gamma-1 chains are ubiquitously expressed, tissue-specificity is provided only by the alpha-2 chain, formerly described as merosin.

Laminin-alpha 2 is widely expressed in the basement membrane of skeletal muscle, at neuromuscular synpases, extra-synaptically and in the myotendinous junctions. LAMA2 has been shown to bind to dystroglycan, which interacts with dystrophin and extra-cellular laminin. Recently mutations have been mapped to the LAMA2 gene that seem imply a role in congenital muscular dystrophy (CMD). {http://jmg.bmjjournals.com/cgi/content/full/38/10/649}

• Positive Control
• Cell Culture

Suitable for use in ELISA, production of anti-serum, and neurite-stimulation assays, and cell attachment assays. Active in cell attachment assays at 10 μg/mL.

Optimal working dilutions must be determined by end user.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene.

FUNCTION: SwissProt: P24043 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
SIZE: 3110 amino acids; 342771 Da
SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-2 is a subunit of laminin-2 (merosin) and laminin-4 (S- merosin).
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
TISSUE SPECIFICITY: Placenta, striated muscle, peripheral nerve, cardiac muscle, pancreas, lung, spleen, kidney, adrenal gland, skin, testis, meninges, choroid plexus, and some other regions of the brain; not in liver, thymus and bone.
DOMAIN: SwissProt: P24043 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI, IV and G are globular.
DISEASE: SwissProt: P24043 # Defects in LAMA2 are the cause of merosin-deficient congenital muscular dystrophy type 1A (MDC1A) [MIM:607855]. MDC1A is characterized by difficulty walking, hypotonia, proximal weakness, hyporeflexia, and white matter hypodensity on MRI.
SIMILARITY: Contains 17 laminin EGF-like domains. & Contains 5 laminin G-like domains. & Contains 2 laminin IV type A domains. & Contains 1 laminin N-terminal domain.

Human

• Human Embryonic Stem Cells
• Mesenchymal Stem Cells
• Neural Stem Cells
• Hematopoietic Stem Cells
• Epithelial Cells
• Pancreatic Stem Cells
• Induced Pluripotent Stem Cells

• Maintenance & Expansion
• General Cell Culture
• Differentiation

Chemicon

Human placenta, assayed negative for antibodies to HBsAg, HIV-1, HIV-2.

Purified merosin protein in PBS containing 0.1% sodium azide.

P24043

Maintain short term at -20ºC in undiluted aliquots for up to three months from date of receipt. For long term storage, maintain at -70ºC in undiluted aliquots for up to one year from date of receipt. Avoid repeated freeze/thaw cycles.

• Stem Cell Reagents
• Attachment Factors

Extracellular Matrix Proteins

Human Merosin

Laminin-2; Laminin M Chain

249μg/mL