Laminin, mouse purified
Description:
Laminin, mouse purified
Trade Name:
Chemicon (Millipore)
Product Overview:
Specificity:Molecular weight = 850 kD; 3 chains expressed as two major bands at 200 and 400 kD on SDS-PAGE.
Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.
Preparation: Prepared from Engelbreth-Holm-Swarm {EHS} sarcoma according to the method of Templ et al 1979 {http://www.jbc.org/cgi/reprint/254/19/9933.pdf} Briefly, tumor homogenates were subjected to NaCl extractions and centrifugations. Laminin was found in the 0.5M NaCl fraction. After subsequent salt gradients and further centrifugation steps, the laminin was dialyzed against 2M urea and passed over a column of DEAE, followed by molecular sieve chromatography and concentration by ultrafiltration.
Key Applications:
- Positive Control
- Cell Culture
Application Notes:
Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue. The optimal laminin concentration may vary with the cell type and has to be determined experimentally.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type:
- Stem Cell Reagents
- Attachment Factors
Concentration:
1 mg/mL
Stem Cell Workflow Stage:
Maintenance & Expansion
Gene Symbol:
- LAMB1
- MGC142015
- CLM
Presentation:
Purified protein. Liquid; in 0.05 M Tris-HCl, pH 7.4, containing 0.15 M NaCl.
No preservatives.
No preservatives.
Stem Cell Type:
- Human Embryonic Stem Cells
- Mouse Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Progenitor Cells
- Pancreatic Stem Cells
Purity:
>95% by SDS-PAGE
PURIFICATION:
Purified as laminin-nidogen complex from EHS sarcoma according to the method of Timpl, et al. (1,2).
PURIFICATION:
Purified as laminin-nidogen complex from EHS sarcoma according to the method of Timpl, et al. (1,2).
Protein or Enzyme Type:
Extracellular Matrix Proteins
Storage Conditions:
Store at -20°C for up to six months.
Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.
Thaw product carefully at 2-8°C to avoid premature gelling, and do not refreeze. Once thawed. material should be stored at 2-8°C for up to 3 months.
Species:
Mouse
Source:
Isolated from Engelbreth-Holm-Swarm (EHS) mouse sarcoma