Autophagy Detection Reagent Pack
Description:
Autophagy Detection Reagent Pack
Qty/Pk:
100 Tests
Product Overview:
Millipore’s Autophagy Detection Reagent Pack provides all of the required reagents and buffers to perform the selective permeabilization technique for the study of autophagy by flow cytometry. This reagent pack is designed to be used with the current Autophagy reporter cell line kits listed as items 1-3 in the related products section below. The Autophagy Detection Reagent Pack extends the usage of the reporter cell lines in these kits, once the original reagent pack is finished. Thus it provides a very cost-effective format.
Background Information:
Autophagy is an intracellular catabolic pathway which causes cellular protein and organelle turnover, and is associated with a diverse range of diseases including Alzheimer's disease, aging-related processes, cancers, and Crohn's disease. It is a tightly regulated process that plays a normal part in cell growth, development, and cellular homeostasis. Autophagy functions as a housekeeping mechanism through disposal of aging and/or dysfunctional proteins and organelles by sequestering and priming these proteins for lysosomal degradation. Increasing evidence suggests that autophagy, in addition to apoptosis, can contribute to cell death and greatly influence general cell health. Malfunctions of the autophagy process are hypothesized to influence cell health, cellular longevity and the ability of cells to function at full capacity.
Key Applications:
Flow Cytometry
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Components:
- 1. Autophagy reagent A: (Part No. CS208212) One vial (lyophilized)
- 2. Autophagy reagent B: (Part No. CS208215) One vial containing 1 mL
- 3. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL
Format:
Cell Line
Storage Conditions:
All reagents must be stored at 2 - 8ºC. All kit components are stable up to six (6) months from date of receipt if stored and handled correctly. Please avoid exposure of the product to repeated changes in temperature as this will affect its integrity.
Detection Methods:
Fluorescent
Product Name:
Autophagy Detection Reagent Pack
Alternate Names:
FlowCellect
Flow Cytometry Software Type:
inCyte
Flow Cytometry Market:
Research