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Rat Brain
Description:
Rat Brain
Trade Name:
Chemicon (Millipore)
Qty/Pk:
500 µg
Product Overview:
Ready to use tissue protein extract from rat brain. Total cellular proteins were isolated from rat brain tissue (Laemmli, 1970; Harlow & lane, 1988).
Key Applications:
Western Blotting
Application Notes:
SDS-PAGE (one or two dimensional)
Isoelectric focusing
Western blotting
Immunoprecipication
Sample preparation: Gel Electrophoresis
Thaw the lysate at room temperature. Avoid freeze/thaw cycles. Heat the sample at 75°C -95°C for 5 minutes to redissolve the SDS, cool slightly. If desired, add DTT or beta-mercaptoethanol to 65mM. Cool on ice. Aliquot desired amount of the protein for loading on SDS-PAGE gels. The amount of protein loaded should be optimized. Should the sample need to be diluted further use 2x Sample Buffer (Laemmli, UK). Dilute 1:1 with 2x Sample Buffer.
Isoelectric focusing
Western blotting
Immunoprecipication
Sample preparation: Gel Electrophoresis
Thaw the lysate at room temperature. Avoid freeze/thaw cycles. Heat the sample at 75°C -95°C for 5 minutes to redissolve the SDS, cool slightly. If desired, add DTT or beta-mercaptoethanol to 65mM. Cool on ice. Aliquot desired amount of the protein for loading on SDS-PAGE gels. The amount of protein loaded should be optimized. Should the sample need to be diluted further use 2x Sample Buffer (Laemmli, UK). Dilute 1:1 with 2x Sample Buffer.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Lysate Type:
Tissue Lysate
Species:
Rat
Quality Assurance:
Protein concentration from the sample was measured by taking absorbency at 280 nm using BSA. Sample was run on a 4-20% polyacrylamide gel. After electrophoresis the gel was stained with coomassie blue and examined for sharp, well resolved protein bands. There was no smeariness indicating no protein degradation. The protein was transferred to PVDF membrane and further tested for the separation and integrity of proteins by using an actin antibody.
Brand Family:
Chemicon
Source:
Rat
Format:
Purified
Presentation:
The lysates with dye are in Laemmli buffer with the following components : Tris Base: 12.11g/lL {0.1M, pH 6.8}
SDS :4% (w/v),{40.0g/L}, Bromophenol Blue 0.2%(w/v) {2.0g/L}, Glycerol 20% (v/v), {200ml/L in molecular biology grade water}.
SDS :4% (w/v),{40.0g/L}, Bromophenol Blue 0.2%(w/v) {2.0g/L}, Glycerol 20% (v/v), {200ml/L in molecular biology grade water}.
Storage Conditions:
Maintain at -80°C in undiluted aliquots for up to six months after date of receipt. Avoid repeated freeze/thaw cycles.
Reagent Type:
Western Blotting Reagents
Product Name:
Rat Brain
Concentration:
2 mg/mL