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Human Prostate
Description:
Human Prostate
Trade Name:
Chemicon (Millipore)
Qty/Pk:
250 µg
Product Overview:
Ready to use tissue protein extract from human prostate gland. Total cellular proteins were isolated from human prostate gland tissue (Laemmli, 1970; Harlow & lane, 1988). Note: Source material has been tested and found to be free from HIV and Hepatitis by PCR.
Key Applications:
Western Blotting
Application Notes:
Gel Electrophoresis:
Thaw the lysate at room temperature. Avoid freeze/thaw cycles.Heat the sample at 75°C -95°C for 5 minutes. Cool on ice. Aliquot desired amount of the protein for loading on SDS-PAGE gels. The amount of protein loaded should be optimized. Should the sample need to be diluted further use 2x Sample Buffer {Laemmli, UK (1970) Cleavage and structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685}. Dilute 1:1 with 2x Sample Buffer.
Thaw the lysate at room temperature. Avoid freeze/thaw cycles.Heat the sample at 75°C -95°C for 5 minutes. Cool on ice. Aliquot desired amount of the protein for loading on SDS-PAGE gels. The amount of protein loaded should be optimized. Should the sample need to be diluted further use 2x Sample Buffer {Laemmli, UK (1970) Cleavage and structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685}. Dilute 1:1 with 2x Sample Buffer.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Lysate Type:
Tissue Lysate
Species:
Human
Quality Assurance:
Protein concentration from the sample was measured by taking absorbency at 280 nm using BSA. Sample was run on a 4-20% polyacrylamide gel. After electrophoresis the gel was stained with coomassie blue and examined for sharp, well resolved protein bands. There was no smeariness indicating no protein degradation. The protein was transferred to PVDF membrane and further tested for the separation and integrity of proteins by using an actin antibody.
Brand Family:
Chemicon
Source:
Human
Format:
Purified
Presentation:
Homogenized protein extract in 1X Laemmli buffer {62.5 mM Tris-HCL, pH 6.8, 25% glycerol, 2% SDS, .35M DTT, 0.01% Bromophenol blue}.
Storage Conditions:
Maintain at -70°C in undiluted aliquots for up to six months after receipt. Avoid repeated freeze/thaw cycles.
Reagent Type:
Western Blotting Reagents
Product Name:
Human Prostate
Concentration:
10 μg/μL