PCT Large Airway Epithelium Medium (Rat)
Description:
PCT Large Airway Epithelium Medium (Rat)
Trade Name:
Chemicon (Millipore)
Product Overview:
PCT Large Airway Epithelium Medium Complete, catalog number CnT-15, is a progenitor cell targeted liquid culture medium kit including both basal medium and separate supplements. CnT-15 is a fully defined, serum and BPE-free formulation, and developed for maximum efficiency isolation and growth of large airway epithelium. It does not contain antibiotics / antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.
Key Applications:
Cell Culture
Application Notes:
Developed for maximum efficiency isolation and growth of large airway epithelium. For suggested seeding densities, please refer to "Suggested Seeding Densities for CELLnTEC Media" in protocols tab. Formulated for rat epithelium, but may be used for other species as well. PCT media are intended for isolation and growth of progenitor cells, and are highly effective in delaying the onset of differentiation. For differentiation applications, the use of a non-PCT formulation, CnT-35, is recommended.
Antibiotics/ Antimycotics may be added if desired; CELLnTEC products CnT-ABM10, CnT-ABM20 or CnT-ABM will provide a final concentration of 100 U/mL Penicillin, 100 µg/mL Streptomycin and 250 ng/mL Amphotericin B.
This medium is designed for use in a 5% CO2 atmosphere.
For suggested cell culture protocols, please see www.cellntec.com.
Antibiotics/ Antimycotics may be added if desired; CELLnTEC products CnT-ABM10, CnT-ABM20 or CnT-ABM will provide a final concentration of 100 U/mL Penicillin, 100 µg/mL Streptomycin and 250 ng/mL Amphotericin B.
This medium is designed for use in a 5% CO2 atmosphere.
For suggested cell culture protocols, please see www.cellntec.com.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
Progenitor Cell Targeting Large Airway Epithelium Medium Complete
Cell Culture Reagent Type:
Cell Culture Media
Media Form:
Liquid
Stem Cell Workflow Stage:
- Cells/Isolation
- Maintenance & Expansion
Media Type:
Cell Culture Media
Components:
- Basal Media (CnT-15.BM), 500mls
- Supplement (CnT-15.S); Containing three Supplements (A, B and C), frozen (To be added to 500 mL basal medium prior to use)
Stem Cell Type:
Epithelial Progenitor Cells
Quality Assurance:
Certified as free of bacteria, fungi, and mycoplasma.
Sales Restrictions:
This product is not available for sale in Japan.
Cell Culture Media or Reagent Type:
Epithelial Progenitor Cell Culture
Storage Conditions:
Basal Media (CnT-15.BM): Store at 4°C in the dark. See label for expiration date.
Supplement (CnT-15.S): Store at -20°C. See label for expiration date.
Supplemented medium: Store at 4°C in the dark Use within 3 months of supplementation. Do not freeze.
Supplement (CnT-15.S): Store at -20°C. See label for expiration date.
Supplemented medium: Store at 4°C in the dark Use within 3 months of supplementation. Do not freeze.