Large Airway Epithelium Medium (rat)
Description:
Large Airway Epithelium Medium (rat)
Trade Name:
Chemicon (Millipore)
Product Overview:
CnT-35 is a liquid culture medium kit, developed for culture of large airway epithelium, including both basal medium and separate supplements. CnT-35 is a fully defined, low calcium, serum and BPE-free formulation which contains 10nM retinoic acid. It does not contain antibiotics / antimycotics*. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.
Key Applications:
Cell Culture
Application Notes:
Developed for culture of large airway epithelium. Cells should be transferred from CnT-15 medium to CnT-35 medium prior to induction of differentiation of large airway epithelium. For suggested seeding densities, please refer to "Suggested Seeding Densities for CELLnTEC Media" in protocols tab. Differentiation may require the addition of 1mM calcium. See protocols for more information.
Antibiotics/ Antimycotics may be added if desired; CELLnTEC products CnT-ABM10, CnT-ABM20 or CnT-ABM will provide a final concentration of 100 U/mL Penicillin, 100 µg/mL Streptomycin and 250 ng/mL Amphotericin B.
This medium is designed for use in a 5% CO2 atmosphere.
Antibiotics/ Antimycotics may be added if desired; CELLnTEC products CnT-ABM10, CnT-ABM20 or CnT-ABM will provide a final concentration of 100 U/mL Penicillin, 100 µg/mL Streptomycin and 250 ng/mL Amphotericin B.
This medium is designed for use in a 5% CO2 atmosphere.
Species Reactivity:
Rat
Species Reactivity Note:
Formulated for rat epithelium, but may be used for other species as well.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type:
Cell Culture Media
Media Form:
Liquid
Stem Cell Workflow Stage:
- Differentiation
- Maintenance & Expansion
Media Type:
Cell Culture Media
Components:
- Basal media, 500 mls (CnT-35.BM)
- Media Supplement (CnT-35.S) Containing three Supplements (A, B and retinoic acid), frozen (To be added to 500 mL basal medium prior to use)
Stem Cell Type:
Epithelial Progenitor Cells
Sales Restrictions:
This product is not available for sale in Japan.
Cell Culture Media or Reagent Type:
Epithelial Progenitor Cell Culture
Storage Conditions:
Basal Media (CnT-35.BM): Store at 4°C in the dark. See label for expiration date.
Supplement (CnT-35.S): Store at -20°C. See label Supplemented medium without retinoic acid: Store at 4°C in the dark. Use within 3 months of supplementation. Do not freeze. Supplemented medium with retinoic acid: Store at 4°C, completely protected from light and with minimized oxygen exposure (store in full containers). Use within 1 month of supplementation. Do not freeze.
Supplement (CnT-35.S): Store at -20°C. See label Supplemented medium without retinoic acid: Store at 4°C in the dark. Use within 3 months of supplementation. Do not freeze. Supplemented medium with retinoic acid: Store at 4°C, completely protected from light and with minimized oxygen exposure (store in full containers). Use within 1 month of supplementation. Do not freeze.