Ordering Information
- : CYL3038
- : 2 mL
PrecisION hERG-CHO Recombinant Cell Line
Description:
PrecisION hERG-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing the human ERG (ether-a-go-go related gene) potassium channel.
Background Information:
The FDA, ICH S7B guidelines set out a non-clinical testing strategy to assess the effects of pharmaceuticals on ventricular repolarisation and proarrhythmic risk. The most common mechanism for this is inhibition of the delayed rectified potassium current IKr. The human ether-à-go-go-related gene (hERG) encodes the potassium ion channel responsible for IKr.
Application Notes:
CHO-K1 cells expressing hERG were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques. The cell-line was shown to be selectively expressing hERG current since the isochronal and fully activated I/V plots were the same as those reported in the literature. Furthermore, tail currents were significantly blocked by low nM concentrations of E4031 and cisapride. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 32 passages: 96.6 % of cells expressed hERG currents at passage 32 giving a mean tail current amplitude at -50 mV of 893 ± 19 pA (n=341).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified.
UniProt Summary:
FUNCTION: SwissProt: Q12809 # Pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.
SIZE: 1159 amino acids; 126655 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH6/ERG2 and KCNH7/ERG3. Interacts with ALG10B (By similarity). Heteromultimer with KCNE1 and KCNE2.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in heart and brain.
DOMAIN:SwissProt: Q12809 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
PTM: Phosphorylated on serine and threonine residues. Phosphorylation by PKA inhibits ion conduction.
DISEASE: SwissProt: Q12809 # Defects in KCNH2 are the cause of long QT syndrome type 2 (LQT2) [MIM:152427]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2. & Defects in KCNH2 are the cause of short QT syndrome type 1 (SQT1) [MIM:609620]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.
SIMILARITY: Belongs to the potassium channel family. H (Eag) subfamily. & Contains 1 cyclic nucleotide-binding domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 1 PAS (PER-ARNT-SIM) domain.
SIZE: 1159 amino acids; 126655 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with KCNH6/ERG2 and KCNH7/ERG3. Interacts with ALG10B (By similarity). Heteromultimer with KCNE1 and KCNE2.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in heart and brain.
DOMAIN:SwissProt: Q12809 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
PTM: Phosphorylated on serine and threonine residues. Phosphorylation by PKA inhibits ion conduction.
DISEASE: SwissProt: Q12809 # Defects in KCNH2 are the cause of long QT syndrome type 2 (LQT2) [MIM:152427]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2. & Defects in KCNH2 are the cause of short QT syndrome type 1 (SQT1) [MIM:609620]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.
SIMILARITY: Belongs to the potassium channel family. H (Eag) subfamily. & Contains 1 cyclic nucleotide-binding domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 1 PAS (PER-ARNT-SIM) domain.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hERG
UniProt Number:
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- KCNH2
- ERG1
- LQT2
- erg1
- HERG
- SQT1
- H-ERG
- Kv11.1
- HERG1
- Erg1
- ERG
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hERG-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- KCNH2
- ERG1
- LQT2
- erg1
- HERG
- SQT1
- H-ERG
- Kv11.1
- HERG1
- Erg1
- ERG