PrecisION hnAChR α7/ric3-HEK Recombinant Cell Line
Description:
PrecisION hnAChR α7/ric3-HEK Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant HEK293 cell line expressing the human nAChR α7/ric3 ligand gated ion channel
Background Information:
α7 nicotinic acetylcholine (nACh7) receptors are predominantly expressed in the CNS, including the hippocampus, cortex and ventral tegmental area. Nicotinic ACh7 receptors are involved with LTP, memory and attention deficits and so are drug development targets for diseases of cognition including Alzheimer’s and schizophrenia. The nACh7 receptors are rapidly desensitized, have homopentameric architecture and respond to agonists including nicotine, acetylcholine, AR-R17779 and epibatidine. In addition the currents are blocked by the competitive antagonist methyllycaconitine. Drug discovery efforts have also identified positive allosteric modulators such as PNU-120596 which increase the potency of activators such as acetylcholine without causing desensitization. This cell line also expresses the ric3 chaperone protein as when co-expressed with the nACh7 receptor, ric-3 significantly increases surface expression.
Application Notes:
HEK cells expressing hnACh7/ric3 channels were characterized in terms of their pharmacological properties using whole-cell PatchXpress® , Port-a-Patch® and FLIPRTETRA™ instruments. The cell line was shown to be selectively expressing hACh7 currents since inward currents were seen upon exposure to acetylcholine and epibatidine in the extracellular solution in cells held at -40 mV. These currents were blocked by methyllycaconitine, a specific hnACh7 channel antagonist. Functional channel expression over time was monitored using the PatchXpress® platform. Channel expression is robust over at least 28 passages as >90% of the cells expressed hnACh7 currents >4 nA at passage 28 in the presence of acetylcholine and PNU-120596.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
HEK
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
293 cell line
Presentation:
2 x 1 ml aliquots
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hnAChR α7/ric3-HEK Recombinant Cell Line