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  • : CYT306
  • : 1 kit

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ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA

Description:
ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA
Trade Name:
  • ChemiKine
  • Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
ChemiKine™ Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. The kit will measure BDNF from human and rat.

Test Principle:

With the ChemiKine BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin-enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration: i.e., the higher the OD the higher the BDNF concentration in the sample.

Application:

ChemiKine BDNF kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended.

For Research Use Only. Not for use in diagnostic procedures.
Applications:
This ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA is used to measure & quantify BDNF levels in Neuroscience research.
Key Applications:
ELISA
Species Reactivity:
  • Human
  • Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene, but the full-length nature of only some could be determined.
UniProt Summary:
FUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
SIZE: 247 amino acids; 27818 Da
SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
SUBCELLULAR LOCATION: Secreted.
PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.
Brand Family:
Chemicon
Storage Conditions:
Precautions:

· Wash Buffer and Sample Buffer contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

· The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.
Gene Symbol:
  • BDNF
  • Abrineurin
  • neurotrophin
  • MGC34632
Product Name:
ChemiKine Brain Derived Neurotrophic Factor, Sandwich ELISA
Alternate Names:
Materials Required but Not Delivered:
1. Multi-channel or repeating pipettes

2. Plate shaker (optional)

3. Pipettors & tips capable of accurately measuring 10-1000 mL

4. Graduated serological pipettes

5. 96-well microplate Reader with 450 nm filter

6. Graph paper for manual plotting of data

7. Polystyrene test tubes for standard and sample dilutions

8. Mechanical vortex

9. One 1 or 2 liter container
Analytes Available:
Components:
  • ChemiKine BDNF ELISA Plate: (Part No. 60238) Two Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.
  • Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.
  • Standard/Sample Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).
  • BDNF Standard (Recombinant Human): (Part No. 60237) Two vials (Lyophilized).
  • Biotinylated Mouse anti-Human BDNF Monoclonal Antibody: (Part No. 60583) One 25 μL vial.
  • Streptavidin-Enzyme conjugate: (Part No. 60582) One 50 mL vial of HRP conjugated Streptavidin.
  • TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.
  • Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution.
UniProt Number:
Detection Methods:
Chromogenic
Entrez Gene Number:

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